Prohaska J R, Bailey W R, Lear P M
Department of Biochemistry and Molecular Biology, University of Minnesota, Duluth 55812, USA.
J Nutr. 1995 Jun;125(6):1447-54. doi: 10.1093/jn/125.6.1447.
Perinatal copper deficiency was studied in 1-mo-old female and male Sprague-Dawley rat offspring to investigate changes in cuproenzymes. Offspring of dams given the low Cu treatment beginning at d 7 of gestation exhibited signs characteristic of Cu deficiency, including a 90% reduction in liver Cu levels compared with Cu-adequate controls. Compared with Cu-adequate rats, Cu-deficient rats had lower activities of the cuproenzymes peptidylglycine alpha-amidating monooxygenase (PAM), cytochrome c oxidase (CCO), and Cu,Zn-superoxide dismutase (SOD) in heart and midbrain samples. Activity of dopamine-beta-monooxygenase (DBM) was higher in midbrain and lower in heart samples from Cu-deficient compared with Cu-adequate rats. Following 1 mo of Cu repletion, PAM and CCO activity were still lower in heart of Cu-replete rats. Midbrain DBM activity was still elevated in the former Cu-deficient males. A second study was conducted using weanling male Holtzman rats. After 5.5 wk of treatment, Cu-deficient rats had signs characteristic of Cu deficiency and lower PAM, CCO and DBM activities in heart but not midbrain as compared with Cu-adequate rats. The PAM activity was lower following Cu deficiency. Perhaps neuropeptide maturation is compromised by Cu deficiency.
对1月龄的雌性和雄性斯普拉格-道利大鼠后代进行围产期铜缺乏研究，以调查铜酶的变化。从妊娠第7天开始接受低铜处理的母鼠所产后代表现出铜缺乏的特征性症状，与铜充足的对照组相比，肝脏铜水平降低了90%。与铜充足的大鼠相比，铜缺乏的大鼠心脏和中脑样本中的肽基甘氨酸α-酰胺化单加氧酶（PAM）、细胞色素c氧化酶（CCO）和铜锌超氧化物歧化酶（SOD）等铜酶活性较低。与铜充足的大鼠相比，铜缺乏大鼠中脑的多巴胺-β-单加氧酶（DBM）活性较高，而心脏中的活性较低。在补充铜1个月后，铜充足的大鼠心脏中PAM和CCO活性仍然较低。在先前铜缺乏的雄性大鼠中，中脑DBM活性仍然升高。使用断奶雄性霍尔茨曼大鼠进行了第二项研究。经过5.5周的处理，与铜充足的大鼠相比，铜缺乏的大鼠出现了铜缺乏的特征性症状，心脏中PAM、CCO和DBM活性较低，但中脑没有。铜缺乏后PAM活性较低。也许神经肽成熟受到铜缺乏的影响。
