State Key Laboratory of Microbial Resources at Institute of Microbiology, CAS, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Microbial Resources at Institute of Microbiology, CAS, Beijing 100101, China.
J Hazard Mater. 2016 Nov 15;318:90-98. doi: 10.1016/j.jhazmat.2016.06.055. Epub 2016 Jun 27.
Microbial degradation of polycyclic aromatic hydrocarbons (PAHs) is the primary process of removing PAHs from environments. The metabolic pathway of PAHs in pure cultures has been intensively studied, but cooperative metabolisms at community-level remained to be explored. In this study, we determined the dynamic composition of a microbial community and its metabolic intermediates during fluoranthene degradation using high-throughput metagenomics and gas chromatography-mass spectrometry (GC-MS), respectively. Subsequently, a cooperative metabolic network for fluoranthene degradation was constructed. The network shows that Mycobacterium contributed the majority of ring-hydroxylating and -cleavage dioxygenases, while Diaphorobacter contributed most of the dehydrogenases. Hyphomicrobium, Agrobacterium, and Sphingopyxis contributed to genes encoding enzymes involved in downstream reactions of fluoranthene degradation. The contributions of various microbial groups were calculated with the PICRUSt program. The contributions of Hyphomicrobium to alcohol dehydrogenases were 62.4% in stage 1 (i.e., when fluoranthene was rapidly removed) and 76.8% in stage 3 (i.e., when fluoranthene was not detectable), respectively; the contribution of Pseudomonas were 6.6% in stage 1 and decreased to 1.2% in subsequent stages. To the best of the author's knowledge, this report describes the first cooperative metabolic network to predict the contributions of various microbial groups during PAH-degradation at community-level.
微生物降解多环芳烃（PAHs）是去除环境中 PAHs 的主要过程。纯培养物中 PAHs 的代谢途径已得到深入研究，但群落水平的协同代谢仍有待探索。在这项研究中，我们分别使用高通量宏基因组学和气相色谱-质谱联用（GC-MS）技术，确定了在荧蒽降解过程中微生物群落的动态组成及其代谢中间产物。随后，构建了荧蒽降解的协同代谢网络。该网络表明，分枝杆菌贡献了大多数环羟基化和环裂解双加氧酶，而食烷菌贡献了大多数脱氢酶。微杆菌、农杆菌和鞘氨醇单胞菌有助于参与荧蒽降解下游反应的酶编码基因。通过 PICRUSt 程序计算了各种微生物组的贡献。在第 1 阶段（即荧蒽迅速去除阶段），Hyphomicrobium 对醇脱氢酶的贡献为 62.4%，在第 3 阶段（即荧蒽不可检测阶段）的贡献为 76.8%；在第 1 阶段，假单胞菌的贡献为 6.6%，随后各阶段的贡献下降至 1.2%。据作者所知，本报告描述了第一个协同代谢网络，用于预测群落水平 PAH 降解过程中各种微生物组的贡献。
