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结直肠癌肝转移患者冷冻手术前后循环肿瘤细胞的分析

Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery.

作者信息

Shi Jian, Li Yuan, Liang Shuzhen, Zeng Jianying, Liu Guifeng, Mu Feng, Li Haibo, Chen Jibing, Liu Tongjun, Niu Lizhi

机构信息

a Department of General Surgery , The Second Hospital of Jilin University , Changchun , Jilin , China.

b Fuda Cancer Hospital , Jinan University School of Medicine, Guangzhou Fuda Cancer Institute , Guangzhou , Guangdong , China.

出版信息

Cancer Biol Ther. 2016 Sep;17(9):935-42. doi: 10.1080/15384047.2016.1210731. Epub 2016 Jul 14.

DOI:10.1080/15384047.2016.1210731
PMID:27415969
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5036405/
Abstract

In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 55 patients with colorectal liver metastasis at 1 day before and 7 and 30 d after cryoablation using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR). The number of CTCs decreased significantly with postoperative time (P < 0.01). Delta cycle threshold values for the CTC-related genes CEA, Ep-CAM, CK18 and CK19 increased significantly after cryoablation. Furthermore, the expression of CEA, Ep-CAM, CK18 and CK19 decreased significantly with time after cryoablation (P < 0.01). RT-qPCR and FACS combined with MACS has significant diagnostic and prognostic value for evaluating the efficacy of cryosurgery in patients with advanced colorectal cancer.

摘要

在本研究中，我们测定了结直肠癌肝转移患者冷冻手术前后外周血循环肿瘤细胞（CTC）的数量，以此作为了解任何变化与冷冻手术疗效相关性的参考。使用磁珠分选法（MACS）和荧光激活细胞分选法（FACS）结合实时定量PCR（RT-qPCR），对55例结直肠癌肝转移患者冷冻消融术前1天、术后7天和30天的外周血进行CTC数量及CTC相关基因表达的检测。CTC数量随术后时间显著减少（P<0.01）。冷冻消融术后，CTC相关基因癌胚抗原（CEA）、上皮细胞黏附分子（Ep-CAM）、细胞角蛋白18（CK18）和细胞角蛋白19（CK19）的Δ循环阈值显著升高。此外，冷冻消融术后，CEA、Ep-CAM、CK18和CK19的表达随时间显著降低（P<0.01）。RT-qPCR以及FACS与MACS相结合，对评估晚期结直肠癌患者冷冻手术的疗效具有显著的诊断和预后价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123d/5036405/5dd3dc8aee82/kcbt-17-09-1210731-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123d/5036405/5a33576d00aa/kcbt-17-09-1210731-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123d/5036405/958498e89642/kcbt-17-09-1210731-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123d/5036405/8f6dd7dfc22c/kcbt-17-09-1210731-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123d/5036405/339447debeb3/kcbt-17-09-1210731-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123d/5036405/5dd3dc8aee82/kcbt-17-09-1210731-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123d/5036405/5a33576d00aa/kcbt-17-09-1210731-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123d/5036405/958498e89642/kcbt-17-09-1210731-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123d/5036405/8f6dd7dfc22c/kcbt-17-09-1210731-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123d/5036405/339447debeb3/kcbt-17-09-1210731-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123d/5036405/5dd3dc8aee82/kcbt-17-09-1210731-g005.jpg

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