Takano Y, Matsuo S, Wakisaka S, Ichikawa H, Nishikawa S, Akai M
Department of Oral Anatomy II, Hokkaido University School of Dentistry, Sapporo, Japan.
Acta Anat (Basel). 1989;134(4):305-11. doi: 10.1159/000146707.
The localization of calcium in the enamel organ of rapidly-frozen, freeze-substituted rat incisors in early-stage amelogenesis was examined by a histochemical calcium-staining method. In secretory ameloblasts, glyoxal bis(2-hydroxyanil) (GBHA) staining revealed intense red reactions in mitochondria and tubulovesicular structures located throughout the cytoplasm, while no reaction was seen in the nucleus and cytosol, nor along the plasma membranes of the respective cells. No significant GBHA reaction was observed in the intercellular compartment and other cells of the enamel organ. Some granular reactions were localized in the cells of the adjacent connective tissue. Control tests confirmed the specificity of GBHA reactions for calcium. Thus, the present observations provide histochemical evidence indicating an exclusive localization of calcium in mitochondria and tubulovesicular structures of the secretory ameloblast, and support their contributions to the translocation of calcium from the proximal to the distal pole of the cytoplasm.
采用组织化学钙染色法，对快速冷冻、冷冻置换的大鼠切牙早期釉质发生过程中牙釉质器官内钙的定位进行了研究。在分泌期成釉细胞中，乙二醛双（2-羟基苯胺）（GBHA）染色显示，线粒体和遍布细胞质的微管泡结构呈现强烈的红色反应，而细胞核、细胞质溶胶以及各细胞的质膜均未见反应。在牙釉质器官的细胞间区和其他细胞中未观察到明显的GBHA反应。相邻结缔组织细胞中有一些颗粒状反应。对照试验证实了GBHA反应对钙的特异性。因此，本研究结果提供了组织化学证据，表明钙在分泌期成釉细胞的线粒体和微管泡结构中具有独特定位，并支持它们在将钙从细胞质近端转运至远端过程中的作用。
