Takano Y, Akai M
First Department of Oral Anatomy, Osaka University, Faculty of Dentistry, Japan.
Histochemistry. 1988;89(5):429-36. doi: 10.1007/BF00492598.
Localization of calcium in a rapid frozen and freeze substituted duodenum of normal, starved or calcium-repleted rat was examined using either of the glyoxal bis(2-hydroxyanil) (GBHA) staining method, a sensitive histochemical calcium stain or electron microscopy. In normally-fed rats, a majority of absorptive cells of the duodenum showed numerous discrete red granular GBHA reactions, approximately 1 micron or less in diameter, located primarily along their lateral plasma membranes and within intercellular spaces. Electron microscopy also revealed electron-dense granules, 30-100 nm in diameter, showing a similar distribution as the GBHA granules in the respective absorptive cells, and confirmed their absence in mitochondria and other intracellular compartments. Some of the absorptive cells located exclusively at the tip of each villus contained highly GBHA-reactive tubulo-vesicular structures extending throughout the cytoplasm. However, they displayed virtually no granular GBHA reaction. In these cells, electron microscopy revealed numerous electron-dense granules in the nucleus, mitochondria and in other unidentified organelles. X-ray microprobe analyses of ultrathin sections confirmed the presence of calcium within electron-dense granules associated with both types of absorptive cells. The number and intensity of all GBHA reactions fluctuated according to luminal calcium concentration. In calcium-repleted rats, strong GBHA reactions appeared in a narrow zone of lamina propria at the tip of the villus, overlaid, predominantly, with absorptive cells showing tubulo-vesicular GBHA reactions. These results suggest the existence of distinct types of absorptive epithelial cells in the rat duodenum, with respect to patterns of calcium localization which they display.
使用乙二醛双（2-羟基苯胺）（GBHA）染色法（一种灵敏的组织化学钙染色法）或电子显微镜，对正常、饥饿或补钙大鼠快速冷冻和冷冻置换后的十二指肠中的钙进行定位研究。在正常喂食的大鼠中，十二指肠的大多数吸收细胞显示出许多离散的红色颗粒状GBHA反应，直径约1微米或更小，主要位于其外侧质膜和细胞间隙内。电子显微镜还显示出直径为30-100纳米的电子致密颗粒，在各自的吸收细胞中显示出与GBHA颗粒相似的分布，并证实它们在线粒体和其他细胞内区室中不存在。一些仅位于每个绒毛顶端的吸收细胞含有高度GBHA反应性的管状小泡结构，贯穿整个细胞质。然而，它们几乎没有颗粒状GBHA反应。在这些细胞中，电子显微镜显示细胞核、线粒体和其他未识别的细胞器中有许多电子致密颗粒。超薄切片的X射线微探针分析证实了与两种类型的吸收细胞相关的电子致密颗粒中存在钙。所有GBHA反应的数量和强度根据管腔钙浓度而波动。在补钙大鼠中，强烈的GBHA反应出现在绒毛顶端固有层的一个狭窄区域，主要覆盖有显示管状小泡GBHA反应的吸收细胞。这些结果表明，就大鼠十二指肠吸收上皮细胞所显示的钙定位模式而言，存在不同类型的吸收上皮细胞。
