Takano Y, Matsuo S, Wakisaka S, Ichikawa H, Nishikawa S, Akai M
First Department of Oral Anatomy, Osaka University Faculty of Dentistry, Japan.
Arch Histol Cytol. 1988 Jul;51(3):241-8. doi: 10.1679/aohc.51.241.
The location of calcium in a rapid-frozen and freeze-substituted maturation stage enamel organ of the rat incisors was demonstrated by means of the glyoxal bis(2-hydroxyanil) (GBHA) staining method, which formed insoluble red precipitates of calcium-GBHA complex. In the ameloblast layer, highly GBHA-reactive tubulo-vesicular structures corresponding to mitochondria and some other membrane-bound structures were localized in both ruffle-ended and smooth-ended ameloblasts, although no significant GBHA reaction was localized in the nucleus, Golgi region, nor along the plasma membrane of these cells. In addition, numerous granular GBHA reactions appeared exclusively in association with the ruffled border of ruffle-ended ameloblasts. GBHA reactions were positive, but were considerably weaker in papillary cells than in the ameloblast. These observations provide a first published histochemical mapping of calcium in the maturation stage enamel organ, and suggest the active participation of mitochondria in maturation stage ameloblasts in calcium regulation.
通过乙二醛双（2-羟基苯胺）（GBHA）染色法证明了大鼠切牙快速冷冻和冷冻替代成熟阶段釉质器官中钙的位置，该方法形成了钙-GBHA复合物的不溶性红色沉淀。在成釉细胞层中，对应于线粒体和其他一些膜结合结构的高度GBHA反应性的管状-囊泡结构位于褶缘和成釉细胞和平滑缘成釉细胞中，尽管在这些细胞的细胞核、高尔基体区域以及质膜上均未发现明显的GBHA反应。此外，大量颗粒状GBHA反应仅出现在褶缘成釉细胞的褶缘处。GBHA反应呈阳性，但乳头细胞中的反应比成釉细胞中的反应弱得多。这些观察结果首次发表了成熟阶段釉质器官中钙的组织化学图谱，并表明线粒体在成熟阶段成釉细胞的钙调节中发挥了积极作用。
