阿拉伯费肯草对凝血酶诱导培养的人脐静脉内皮细胞释放组织型纤溶酶原激活物及纤溶酶原激活物抑制物-1与组织型纤溶酶原激活物复合物的影响。

Effect of Fagonia arabica on thrombin induced release of t-PA and complex of PAI-1 tPA in cultured HUVE cells.

作者信息

Aloni Prutha D, Nayak Amit R, Chaurasia Sweta R, Deopujari Jayant Y, Chourasia Chhaya, Purohit Hemant J, Taori Girdhar M, Daginawala Hatim F, Kashyap Rajpal S

机构信息

Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur 440010, Maharashtra, India.

Matru Sewa Sangh, Maternity Home, Kothi Road, Mahal, Nagpur 440002, Maharashtra, India.

出版信息

J Tradit Complement Med. 2015 Apr 3;6(3):219-23. doi: 10.1016/j.jtcme.2015.03.002. eCollection 2016 Jul.

Fagonia arabica (FA) possesses a thrombolytic property which has been earlier reported in our laboratory. Current study was undertaken to investigate the effect of aqueous extract of FA on thrombin-induced tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) release from cultured human umbilical vein endothelial cell line (HUVE) for studying its clot lytic activity. For this, establishment of cell line model has been done by isolating the cells from human umbilical cord. Cell toxicity was evaluated using XTT assay. Estimation of t-PA and PAI-1 t-PA complex were done using ELISA technique. Thrombin treatment induces the t-PA and PAI-1 release from HUVE cell line, and FA treatment was found to antagonize the thrombin induced t-PA and PAI-1 release. Our preliminary results suggest that FA may be used as an alternative to thrombolytic drug. However, study demands further experiments using animal model of thrombosis to establish the role of FA as a novel thrombolytic drug.

阿拉伯骆驼蓬（FA）具有溶栓特性，此前我们实验室已有相关报道。本研究旨在探讨FA水提取物对凝血酶诱导的组织纤溶酶原激活物（t-PA）和纤溶酶原激活物抑制剂-1（PAI-1）从培养的人脐静脉内皮细胞系（HUVE）释放的影响，以研究其溶栓活性。为此，通过从人脐带中分离细胞建立了细胞系模型。使用XTT法评估细胞毒性。采用ELISA技术测定t-PA和PAI-1/t-PA复合物。凝血酶处理可诱导HUVE细胞系释放t-PA和PAI-1，而FA处理可拮抗凝血酶诱导的t-PA和PAI-1释放。我们的初步结果表明，FA可作为溶栓药物的替代品。然而，该研究需要使用血栓形成动物模型进行进一步实验，以确定FA作为新型溶栓药物的作用。