利用混合微生物培养物从发酵乳清中生产聚羟基烷酸酯（PHA）。
Polyhydroxyalkanoates (PHAs) production from fermented cheese whey by using a mixed microbial culture.
机构信息
Gruppo Ricicla labs - DiSAA - Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy.
REQUIMTE/CQFB, Department of Chemistry, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
出版信息
Bioresour Technol. 2016 Oct;218:692-9. doi: 10.1016/j.biortech.2016.07.024. Epub 2016 Jul 8.
Two fermented cheese wheys (FCW), FCW1 composed of lactic, acetic and butyric acids in the proportion of 58/16/26 (% CODOrganic Acid (OA)) and FCW2 composed of acetic, propionic, butyric, lactic and valeric acids in the proportion of 58/19/13/6/4 (% CODOA) were used to produce polyhydroxyalkanoates (PHAs) by using a pre-selected mixed microbial culture (MMC). PHA accumulation gave for fermented FCW1 a PHA yield (Ytot) of 0.24±0.02mgCODPHAmgCODSolubleSubstrate(SS)(-1) and a total PHA production, referred to the substrate used, of 60gPHAkgcheesewheyTotalSolids(TS)(-1). For fermented FCW2 results were: PHA yield (Ytot) of 0.42±0.03mgCODPHAmgCODSS(-1) and PHA from a substrate of 70gPHAkgcheesewheyTS(-1). Qualitatively, PHAs from FCW1 was made up exclusively of 3-hydroxybutyrate (HB), while those obtained from FCW2 were composed of 40% of 3-hydroxyvalerate (HV) and 60% of HB.
两种发酵奶酪乳清（FCW），FCW1 由乳酸、乙酸和丁酸按 58/16/26（% COD 有机酸（OA））的比例组成，FCW2 由乙酸、丙酸、丁酸、乳酸和缬草酸按 58/19/13/6/4（% CODOA）的比例组成，均用于通过预选择的混合微生物培养物（MMC）生产聚羟基烷酸酯（PHA）。发酵 FCW1 的 PHA 积累给出了 0.24±0.02mgCODPHAmgCODSolubleSubstrate(SS)(-1)的 PHA 产率（Ytot）和 60gPHAkgcheesewheyTotalSolids(TS)(-1)的总 PHA 产量。对于发酵 FCW2 的结果是：PHA 产率（Ytot）为 0.42±0.03mgCODPHAmgCODSS(-1)，底物为 70gPHAkgcheesewheyTS(-1)。定性地说，FCW1 中的 PHAs 仅由 3-羟基丁酸（HB）组成，而 FCW2 获得的 PHAs 由 40%的 3-羟基戊酸（HV）和 60%的 HB 组成。
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