Liang Shao-Hua, Yin Jun-Bin, Sun Yi, Bai Yang, Zhou Kai-Xiang, Zhao Wen-Jun, Wang Wei, Dong Yu-Lin, Li Yun-Qing
Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian 350108, China.
Department of Anatomy, Histology and Embryology and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China.
Neurosci Lett. 2016 Aug 26;629:245-250. doi: 10.1016/j.neulet.2016.07.017. Epub 2016 Jul 14.
Combined the retrograde double tracing with immunofluorescence histochemical staining, we examined the neurons in the lateral parabrachial nucleus (LPB) sent collateral projections to the paraventricular thalamic nucleus (PVT) and central amygdaloid nucleus (CeA) and their roles in the nociceptive transmission in the rat. After the injection of Fluoro-gold (FG) into the PVT and tetramethylrhodamine-dextran (TMR) into the CeA, respectively, FG/TMR double-labeled neurons were observed in the LPB. The percentages of FG/TMR double-labeled neurons to the total number of FG- or TMR-labeled neurons were 6.18% and 9.09%, respectively. Almost all of the FG/TMR double-labeled neurons (95%) exhibited calcitonin gene-related peptide (CGRP) immunoreactivity. In the condition of neuropathic pain, 94% of these neurons showed FOS immunoreactivity. The present data indicates that some of CGRP-expressing neurons in the LPB may transmit nociceptive information toward the PVT and CeA by way of axon collaterals.
结合逆行双重追踪和免疫荧光组织化学染色，我们研究了大鼠外侧臂旁核（LPB）中向丘脑室旁核（PVT）和中央杏仁核（CeA）发出侧支投射的神经元及其在伤害性信息传递中的作用。分别向PVT注射荧光金（FG）和向CeA注射四甲基罗丹明葡聚糖（TMR）后，在LPB中观察到FG/TMR双标记神经元。FG/TMR双标记神经元占FG或TMR标记神经元总数的百分比分别为6.18%和9.09%。几乎所有FG/TMR双标记神经元（95%）都表现出降钙素基因相关肽（CGRP）免疫反应性。在神经性疼痛状态下，这些神经元中有94%表现出FOS免疫反应性。目前的数据表明，LPB中一些表达CGRP的神经元可能通过轴突侧支向PVT和CeA传递伤害性信息。
