Robinson M W, Scott D G, Bacon P A, Walton K W, Coppock J S, Scott D L
Department of Rheumatology, Medical School, University of Birmingham.
Ann Rheum Dis. 1989 Jun;48(6):496-501. doi: 10.1136/ard.48.6.496.
The proteins present in 4% polyethylene glycol (PEG) precipitates of 10 normal sera and 60 samples from patients with rheumatic diseases were studied. A variety of immunochemical methods were used, including estimation of the percentages of total serum proteins precipitated by PEG, gel filtration analyses of the precipitates, and affinity chromatography with protein A and anti-immunoglobulin columns. Substantial amounts of protein were precipitated from normal sera. Many non-immunoglobulin proteins were precipitated from patients' sera, including fibronectin, haptoglobin, albumin, transferrin, and alpha 1-antitrypsin. Affinity chromatography with anti-immunoglobulin columns bound non-immunoglobulin proteins from PEG precipitates, but the protein A affinity column did not do so. The view that circulating antibody-antigen complexes alone are precipitated by 4% PEG is too simplistic; many non-immunoglobulin proteins are involved. They may either bind to immune complexes or be coprecipitated owing to non-specific protein aggregation.
对10份正常血清和60份风湿性疾病患者样本的4%聚乙二醇（PEG）沉淀物中的蛋白质进行了研究。使用了多种免疫化学方法，包括估算PEG沉淀的总血清蛋白百分比、沉淀物的凝胶过滤分析以及用蛋白A和抗免疫球蛋白柱进行亲和层析。正常血清中有大量蛋白质沉淀。患者血清中沉淀出许多非免疫球蛋白蛋白质，包括纤连蛋白、触珠蛋白、白蛋白、转铁蛋白和α1-抗胰蛋白酶。用抗免疫球蛋白柱进行亲和层析能结合PEG沉淀物中的非免疫球蛋白蛋白质，但蛋白A亲和柱则不能。认为仅循环抗体-抗原复合物可被4% PEG沉淀的观点过于简单；许多非免疫球蛋白蛋白质也参与其中。它们可能与免疫复合物结合，或者由于非特异性蛋白质聚集而共沉淀。
