Sun Dali, Pang Junxiao, Zhou Zhiqin, Jiao Bining
Citrus Research Institute, Southwest University, Chongqing 400712, China; College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China.
Citrus Research Institute, Southwest University, Chongqing 400712, China.
Chemosphere. 2016 Oct;161:167-173. doi: 10.1016/j.chemosphere.2016.06.087. Epub 2016 Jul 15.
Enantioselective dissipation behavior of the new acaricide cyflumetofen (CYF) in citrus and soil, and its cytotoxicity to human liver hepatocellular carcinoma (HepG2) cells was investigated for the first time. The results of degradation experiment showed that roughly similar half-lives of (-)-CYF and (+)-CYF were achieved in citrus (16.5 and 19.8 d) and soil (6.37 and 6.99 d), respectively. EF values varied from 0.50 to 0.42 in citrus and from 0.49 to 0.48 in soil, indicating that slightly enantioselective degradation happened during experiment period. Moreover, indexes of MTT, LDH, ROS, MDA, SOD, and CAT were used to evaluate enantioselective cytotoxicity and oxidative stress of CYF enantiomers to HepG2 cells. Dose- and structure form-dependent phenomenon was observed with toxicity orders of (-)-CYF > rac-CYF > (+)-CYF. Despite the similar environmental degradation behavior, the toxicities of CYF enantiomers showed great difference, suggesting that (+)-CYF might be developed as potential substitute of rac-CYF for safety consideration.
首次研究了新型杀螨剂乙螨唑（CYF）在柑橘和土壤中的对映体选择性消解行为及其对人肝癌（HepG2）细胞的细胞毒性。降解实验结果表明，（-）-CYF和（+）-CYF在柑橘中的半衰期大致相似（分别为16.5和19.8天），在土壤中的半衰期也大致相似（分别为6.37和6.99天）。柑橘中的对映体分数（EF）值在0.50至0.42之间，土壤中的EF值在0.49至0.48之间，表明在实验期间发生了轻微的对映体选择性降解。此外，采用MTT、LDH、ROS、MDA、SOD和CAT指标评估CYF对映体对HepG2细胞的对映体选择性细胞毒性和氧化应激。观察到剂量和结构形式依赖性现象，毒性顺序为（-）-CYF > 消旋CYF > （+）-CYF。尽管CYF对映体的环境降解行为相似，但其毒性存在很大差异，从安全性考虑，（+）-CYF可能被开发为消旋CYF的潜在替代品。
