Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China; Academy of Agricultural Sciences, Southwest University, Chongqing, China; State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Southwest University, Chongqing, China.
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China; Academy of Agricultural Sciences, Southwest University, Chongqing, China; State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Southwest University, Chongqing, China.
Sci Total Environ. 2021 Jun 1;771:144831. doi: 10.1016/j.scitotenv.2020.144831. Epub 2021 Jan 27.
Cyflumetofen (CYF) is a novel chiral acaricide widely used in commercial crops to control mites. The environmental risks exposed by CYF in the soil, especially at the enantiomer level, remain unclear. We found that the (+)-CYF enantiomer was preferentially degraded in acid-soil, resulting in (-)-CYF enrichment. 16S rRNA and qPCR analysis indicated that decreased bacterial abundance by 12.79-61.80% and 2.52-52.48% in (-)-CYF treatment and (+)-CYF treatment, respectively. Diversity was also decreased with (-)-CYF treatment. Interestingly, several beneficial bacteria, for instance, Alphaproteobacteria (class), Sphingomonadaceae (family), and Arthrobacter (specise) were more enriched following (-)-CYF. The abundance of N-fixing bacteria showed a sustained reduction with time, and the decrease was 3.24-72.94% with (-)-CYF and 25.37-73.11% with (+)-CYF treatment. Compared with the (+)-CYF treatment could positively promote nitrification, while the treatment (-)-CYF significantly reduced the abundance of amoA gene; namely it significantly negatively affected the nitrification in the nitrogen cycle. Through our further research, we found that Actinobacteria, Alphaproteobacteria, Lysobacter; Sphingomonas, Patescibacteria, Saccharimonadia, and Saccharimonadales showed synergistic effects with the nitrogen cycling-related genes nifH and amoA. These results contribute to a comprehensive environmental risk assessment of CYF in acid-soil at the enantiomer level.
氰氟虫腙（CYF）是一种新型手性杀螨剂，广泛应用于商业作物以控制螨虫。然而，CYF 在土壤中的环境风险，尤其是对映体水平的风险尚不清楚。我们发现，（+）-CYF 对映体在酸性土壤中优先降解，导致（-）-CYF 富集。16S rRNA 和 qPCR 分析表明，（-）-CYF 处理和（+）-CYF 处理分别导致细菌丰度降低 12.79-61.80%和 2.52-52.48%。（-）-CYF 处理也降低了多样性。有趣的是，一些有益细菌，例如，α-变形菌（纲）、鞘氨醇单胞菌科（科）和节杆菌（种）在（-）-CYF 处理后更丰富。固氮菌的丰度随着时间的推移持续减少，（-）-CYF 的减少率为 3.24-72.94%，（+）-CYF 的减少率为 25.37-73.11%。与（+）-CYF 处理相比，（-）-CYF 能显著促进硝化作用，而（-）-CYF 处理则显著降低 amoA 基因的丰度；即它显著负影响了氮循环中的硝化作用。通过进一步研究，我们发现放线菌、α-变形菌、赖氨酸杆菌；鞘氨醇单胞菌、Patescibacteria、Saccharimonadia 和 Saccharimonadales 与氮循环相关基因 nifH 和 amoA 表现出协同作用。这些结果有助于在对映体水平上对 CYF 在酸性土壤中的环境风险进行全面评估。
