合成生物发光腔肠素衍生物

Synthetic Bioluminescent Coelenterazine Derivatives.

作者信息

Nishihara Ryo, Citterio Daniel, Suzuki Koji

机构信息

Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa, 223-8522, Japan.

出版信息

Methods Mol Biol. 2016;1461:19-31. doi: 10.1007/978-1-4939-3813-1_2.

DOI:10.1007/978-1-4939-3813-1_2

PMID:27424892

Abstract

The development of coelenterazine (CTZ) derivatives resulting in superior optical characteristics is an efficient method to extend the range of its possible applications. Here, we describe the synthesis of three C-6 substituted CTZ derivatives retaining the recognition by Renilla luciferase (RLuc) and its derivatives. The novel derivatives are useful as bright blue-shifted CTZ derivatives, which can be used as an alternative to hitherto reported compound DeepBlueC™.

摘要

开发具有卓越光学特性的腔肠素（CTZ）衍生物是拓展其潜在应用范围的有效方法。在此，我们描述了三种C-6位取代的CTZ衍生物的合成，这些衍生物保留了对海肾荧光素酶（RLuc）及其衍生物的识别能力。这些新型衍生物作为亮蓝色偏移的CTZ衍生物很有用，可作为迄今报道的化合物DeepBlueC™的替代品。