Nishihara Ryo, Citterio Daniel, Suzuki Koji
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa, 223-8522, Japan.
Methods Mol Biol. 2016;1461:19-31. doi: 10.1007/978-1-4939-3813-1_2.
The development of coelenterazine (CTZ) derivatives resulting in superior optical characteristics is an efficient method to extend the range of its possible applications. Here, we describe the synthesis of three C-6 substituted CTZ derivatives retaining the recognition by Renilla luciferase (RLuc) and its derivatives. The novel derivatives are useful as bright blue-shifted CTZ derivatives, which can be used as an alternative to hitherto reported compound DeepBlueC™.
开发具有卓越光学特性的腔肠素（CTZ）衍生物是拓展其潜在应用范围的有效方法。在此，我们描述了三种C-6位取代的CTZ衍生物的合成，这些衍生物保留了对海肾荧光素酶（RLuc）及其衍生物的识别能力。这些新型衍生物作为亮蓝色偏移的CTZ衍生物很有用，可作为迄今报道的化合物DeepBlueC™的替代品。
