Department of Medicinal Chemistry, Key Laboratory of Chemical Biology, School of Pharmacy, Shandong University, Jinan, Shandong 250012, China.
Org Biomol Chem. 2018 Jul 4;16(26):4789-4792. doi: 10.1039/c8ob01192c.
To develop a photoactivatable bioluminescence imaging technique, a set of high and efficient photoactivatable substrates for Renilla luciferase has been well designed and synthesized. Surprisingly, all of them could release the free luciferin that presented robust bioluminescent signals ex vivo and in living animals after UV irradiation at 365 nm.
为了开发光激活生物发光成像技术，我们精心设计并合成了一组高效的 Renilla 荧光素光激活底物。令人惊讶的是，所有这些底物在 365nm 的紫外光照射下都能释放出游离的荧光素，在离体和活体动物中产生强烈的生物发光信号。
