Kim Sung-Bae, Naganawa Ryuichi, Murata Shingo, Nakayama Takayoshi, Miller Simon, Senda Toshiya
Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), Onogawa 16-1, Tsukuba, Ibaraki, 305-8569, Japan.
Nishihara Electronics co. ltd, 6-8-30, Nishihara, Kashiwa, Chiba, 277-0885, Japan.
Methods Mol Biol. 2016;1461:279-87. doi: 10.1007/978-1-4939-3813-1_23.
A bioluminescence-based assay system was fabricated for an efficient determination of the activities of air pollutants. The following four components were integrated into this assay system: (1) an 8-channel assay platform uniquely designed for simultaneously sensing multiple optical samples, (2) single-chain probes illuminating toxic chemicals or heavy metal cations from air pollutants, (3) a microfluidic system for circulating medium mimicking the human body, and (4) the software manimulating the above system. In the protocol, we briefly introduce how to integrate the components into the system and the application to the illumination of the metal cationic activities in air pollutants.
构建了一种基于生物发光的检测系统，用于高效测定空气污染物的活性。该检测系统集成了以下四个组件：(1) 专门设计的8通道检测平台，用于同时传感多个光学样本；(2) 可照亮空气污染物中有毒化学物质或重金属阳离子的单链探针；(3) 用于循环模拟人体介质的微流控系统；(4) 模拟上述系统的软件。在该方案中，我们简要介绍了如何将这些组件集成到系统中以及在空气污染物中金属阳离子活性检测方面的应用。
