Kim Sung-Bae, Naganawa Ryuichi
Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), Onogawa 16-1, Tsukuba, Ibaraki, 305-8569, Japan.
Methods Mol Biol. 2016;1461:271-8. doi: 10.1007/978-1-4939-3813-1_22.
The present protocol introduces a multichannel bioluminescence determination platform allowing a high sample throughput determination of weak bioluminescence with reduced standard deviations. The platform is designed to carry a multichannel conveyer, an optical filter, and a mirror cap. The platform enables us to near-simultaneously determine ligands in multiple samples without the replacement of the sample tubes. Furthermore, the optical filters beneath the multichannel conveyer are designed to easily discriminate colors during assays. This optical system provides excellent time- and labor-efficiency to users during bioassays.
本方案介绍了一种多通道生物发光测定平台，该平台能够以较低的标准偏差对微弱生物发光进行高通量样品测定。该平台设计用于搭载多通道传送带、光学滤光片和镜帽。该平台使我们能够在不更换样品管的情况下近乎同时测定多个样品中的配体。此外，多通道传送带下方的光学滤光片设计用于在测定过程中轻松区分颜色。这种光学系统在生物测定过程中为用户提供了出色的时间和劳动效率。
