Iinuma Masataka, Kadoya Yutaka, Kuroda Akio
Graduate school of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, 739-8530, Hiroshima, Japan.
Methods Mol Biol. 2016;1461:299-310. doi: 10.1007/978-1-4939-3813-1_25.
The technique of photon counting is widely used for various fields and also applicable to a high-sensitivity detection of luminescence. Thanks to recent development of single photon detectors with avalanche photodiodes (APDs), the photon counting system with an optical fiber has become powerful for a detection of bioluminescence at an optical fiber end, because it allows us to fully use the merits of compactness, simple operation, highly quantum efficiency of the APD detectors. This optical fiber-based system also has a possibility of improving the sensitivity to a local detection of Adenosine triphosphate (ATP) by high-sensitivity detection of the bioluminescence. In this chapter, we are introducing a basic concept of the optical fiber-based system and explaining how to construct and use this system.
光子计数技术广泛应用于各个领域，也适用于高灵敏度的发光检测。得益于近期雪崩光电二极管（APD）单光子探测器的发展，带有光纤的光子计数系统在检测光纤末端的生物发光方面变得十分强大，因为它使我们能够充分利用APD探测器紧凑、操作简单、量子效率高的优点。这种基于光纤的系统还有可能通过对生物发光的高灵敏度检测来提高对三磷酸腺苷（ATP）局部检测的灵敏度。在本章中，我们将介绍基于光纤系统的基本概念，并解释如何构建和使用该系统。
