结肠癌转移相关蛋白1（MACC1）促进结肠癌细胞的生长和转移过程。
Metastasis associated in colon cancer 1 (MACC1) promotes growth and metastasis processes of colon cancer cells.
作者信息
Tang J, Chen J-X, Chen L, Tang J-Y, Cui Z, Liu C-H, Wang Z
机构信息
Department of General Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
出版信息
Eur Rev Med Pharmacol Sci. 2016 Jul;20(13):2825-34.
OBJECTIVE
Colon cancer (CC) is the third most common malignancy with high mortality rate in the world. The impacts of metastasis associated in colon cancer 1 (MACC1) on growth and metastasis processes of CC were investigated by overexpression and interference lentivirus infection.
MATERIALS AND METHODS
Firstly, overexpression and interference plasmids were constructed with GV115 vector and lentivirus were packaged using 293T cells. Human CC cell lines SW1116 and HCT116 were used and divided into four groups respectively, namely control group, blank group, MACC1 siRNA group (infected with interference lentivirus) and MACC1 group (infected with overexpression lentivirus). Then, cell proliferation and clone formation were examined. Besides, migration and invasion of cells were investigated by wound healing and transwell assays, respectively. Additionally, liver metastasis was evaluated in nude xenografts model.
RESULTS
Overexpression and interference lentivirus of MACC1 were successfully constructed. After infected with overexpression lentivirus, cell proliferation, clone formation number, cell invasion and migration capabilities of CC cells were significantly increased (p < 0.05). Furthermore, decreased cell proliferation, smaller and fewer clones, as well as weakened cell migratory and invasive capabilities were observed in SW1116 and HCT116 cells treated with interference lentivirus of MACC1 (p < 0.05). Additionally, liver metastasis rate was higher in SW1116 cells with a higher expression level of MACC1 than that in HCT116 cells with a lower MACC1 expression level.
CONCLUSIONS
MACC1 promotes the growth and metastasis processes of CC cells.
目的
结肠癌（CC）是全球第三大常见恶性肿瘤，死亡率高。通过过表达和干扰慢病毒感染，研究结肠癌转移相关蛋白1（MACC1）对CC生长和转移过程的影响。
材料与方法
首先，用GV115载体构建过表达和干扰质粒，并用293T细胞包装慢病毒。使用人CC细胞系SW1116和HCT116，分别分为四组，即对照组、空白组、MACC1 siRNA组（感染干扰慢病毒）和MACC1组（感染过表达慢病毒）。然后，检测细胞增殖和克隆形成。此外，分别通过伤口愈合试验和Transwell试验研究细胞的迁移和侵袭。另外，在裸鼠异种移植模型中评估肝转移情况。
结果
成功构建了MACC1的过表达和干扰慢病毒。感染过表达慢病毒后，CC细胞的增殖、克隆形成数量、细胞侵袭和迁移能力显著增加（p<0.05）。此外，用MACC1干扰慢病毒处理的SW1116和HCT116细胞中，细胞增殖减少，克隆更小且数量更少，细胞迁移和侵袭能力减弱（p<0.05）。另外，MACC1表达水平较高的SW1116细胞的肝转移率高于MACC1表达水平较低的HCT116细胞。
结论
MACC1促进CC细胞的生长和转移过程。
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