非小细胞肺癌患者血清中 Rac1 和 Rac1b 的无标记实时检测分析。

Quantification of Rac1 and Rac1b in serum of non small cell lung cancer by label free real time assay.

机构信息

Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.

Department of Pulmonary Medicine and Sleep Disorders, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.

出版信息

Clin Chim Acta. 2016 Sep 1;460:231-5. doi: 10.1016/j.cca.2016.07.009. Epub 2016 Jul 15.

DOI:10.1016/j.cca.2016.07.009

PMID:27425849

Abstract

BACKGROUND

Rac proteins play a major role in tumorogenesis. We quantified Rac1 and Rac1b in serum of non small cell lung cancer (NSCLC) patients.

METHODS

The blood of 77 NSCLC patients and 52 healthy controls were collected and quantified the concentration of Rac1 and Rac1b mainly by surface plasmon resonance and it was verify by Western blot analysis.

RESULTS

Rac1 and Rac1b were found to be significantly over expressed in serum of NSCLC patients compare to healthy controls. The level of Rac proteins were found to be increased in all stages of cancer. Despite the low survival rate, we managed to collect serum sample of the 18 follow up patients after the therapy, where 11 patients' of CR+PR group showed down regulation of the Rac protein after chemotherapy and unfortunately 80% patients died during the study period.

CONCLUSION

The high specificity and sensitivity obtained from ROC analysis for Rac1 and Rac1b envisaged it to be used as a serum diagnostic marker in the early stage of cancer.

摘要

背景

Rac 蛋白在肿瘤发生中起主要作用。我们定量检测了非小细胞肺癌（NSCLC）患者血清中的 Rac1 和 Rac1b。

方法

收集 77 例 NSCLC 患者和 52 例健康对照者的血液，主要通过表面等离子体共振定量检测 Rac1 和 Rac1b 的浓度，并通过 Western blot 分析进行验证。

结果

与健康对照组相比，Rac1 和 Rac1b 在 NSCLC 患者的血清中明显过表达。在所有癌症阶段，Rac 蛋白的水平都有所增加。尽管生存率较低，但我们还是成功地收集了 18 例治疗后随访患者的血清样本，其中 11 例 CR+PR 组患者在化疗后 Rac 蛋白表达下调，不幸的是，80%的患者在研究期间死亡。