Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, Poland.
Faculty of Chemical Technology, Poznan University of Technology, 60-965 Poznan, Poland.
Biosensors (Basel). 2018 Dec 16;8(4):132. doi: 10.3390/bios8040132.
More than 50 papers on surface plasmon resonance biosensors, published between 2016 and mid-2018, are reviewed. Papers concerning the determination of large particles such as vesicles, exosomes, cancer cells, living cells, stem cells, and microRNA are excluded, as these are covered by a very recent review. The reviewed papers are categorized into five groups, depending on the degree of maturity of the reported solution; ranging from simple marker detection to clinical application of a previously developed biosensor. Instrumental solutions and details of biosensor construction are analyzed, including the chips, receptors, and linkers used, as well as calibration strategies. Biosensors with a sandwich structure containing different nanoparticles are considered separately, as are SPR (Surface Plasmon Resonance) applications for investigating the interactions of biomolecules. An analysis is also made of the markers determined using the biosensors. In conclusion, there is shown to be a growing number of SPR applications in the solution of real clinical problems.
对 2016 年至 2018 年年中发表的 50 多篇关于表面等离子体共振生物传感器的论文进行了回顾。排除了关于大颗粒（如囊泡、外泌体、癌细胞、活细胞、干细胞和 microRNA）的测定的论文，因为这些内容在最近的一篇综述中都有涉及。所回顾的论文分为五类，根据所报道的解决方案的成熟程度而定；从简单的标记物检测到以前开发的生物传感器的临床应用。对仪器解决方案和生物传感器构建的细节进行了分析，包括所使用的芯片、受体和连接子，以及校准策略。含有不同纳米颗粒的夹心结构的生物传感器被单独考虑，以及用于研究生物分子相互作用的 SPR（表面等离子体共振）应用。还分析了使用生物传感器测定的标记物。总之，在解决实际临床问题的方案中，SPR 的应用越来越多。
