CPS1在黄曲霉毒素B1诱导的肝癌发生中的生物学功能研究。
Studies on the biological functions of CPS1 in AFB1 induced hepatocarcinogenesis.
作者信息
Yang Chi, Fu Rao, Zhuang Zhenhong, Wang Shihua
机构信息
Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of the Education Ministry, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Institute of Edible Fungi, National and Local Joint Engineering Research Center for Breeding & Cultivation of Featured Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou 350014, China.
Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of the Education Ministry, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
出版信息
Gene. 2016 Oct 10;591(1):255-261. doi: 10.1016/j.gene.2016.07.031. Epub 2016 Jul 15.
Carbamyl phosphate synthetase 1 (CPS1) was down-regulated in hepatocellular carcinoma (HCC), as treated by aflatoxin B1 (AFB1), a potent hepatocarcinogenesis mycotoxin. In this study, we firstly confirmed that AFB1 down-regulated the expression of CPS1 in a dose-dependent manner. At the meantime, both siRNA knock down of CPS1 and AFB1 treatment inhibited cell proliferation, and induced cell apoptosis. To further analysis the function of CPS1, the interacting proteins of CPS1 were searched by Co-IP, and three interacting proteins including type II cytoskeletal 1 (KRT1), albumin (ALB), and ubiquitin C (UBC) were found. Both KRT1 and ALB were new interacting proteins for CPS1. Our further study showed that CPS1 was regulating interacted and colocalized with KRT1 and ALB, and the intensity correlation was changed by AFB1. KRT1, ALB and CPS1 were all reported to play an important role in differentiation and tissue specialization. These results may offer an increasing understand that CPS1 might have a function in differentiation.
氨甲酰磷酸合成酶1（CPS1）在黄曲霉毒素B1（AFB1，一种强效的肝癌发生霉菌毒素）处理的肝细胞癌（HCC）中表达下调。在本研究中，我们首先证实AFB1以剂量依赖的方式下调CPS1的表达。同时，CPS1的siRNA敲低和AFB1处理均抑制细胞增殖并诱导细胞凋亡。为进一步分析CPS1的功能，通过免疫共沉淀法寻找CPS1的相互作用蛋白，发现了三种相互作用蛋白，包括细胞角蛋白1（KRT1）、白蛋白（ALB）和泛素C（UBC）。KRT1和ALB都是CPS1新的相互作用蛋白。我们的进一步研究表明，CPS1与KRT1和ALB相互作用并共定位，且AFB1改变了它们之间的强度相关性。KRT1、ALB和CPS1均被报道在分化和组织特化中起重要作用。这些结果可能有助于进一步理解CPS1可能在分化中发挥作用。
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