Toumi Hela, Bejaoui Mustapha, Touaylia Samir, Burga Perez Karen F, Ferard Jean François
a Laboratoire de Bio-surveillance de l'Environnement (LBE), Université de Carthage, Faculté des Sciences de Bizerte , Zarzouna , Bizerte , Tunisie.
b Laboratoire Interdisciplinaire des Environnements Continentaux, Université de Lorraine , Metz , France.
J Environ Sci Health B. 2016 Nov;51(11):777-80. doi: 10.1080/03601234.2016.1198645. Epub 2016 Jul 18.
The present study was designed to investigate the effect of carbaryl (carbamate insecticide) on the acetylcholinesterase activity in two strains (same clone A) of the crustacean cladoceran Daphnia magna. Four carbaryl concentrations (0.4, 0.9, 1.8 and 3.7 µg L(-1)) were compared against control AChE activity. Our results showed that after 48 h of carbaryl exposure, all treatments induced a significant decrease of AChE activities whatever the two considered strains. However, different responses were registered in terms of lowest observed effect concentrations (LOEC: 0.4 µg L(-1) for strain 1 and 0.9 µg L(-1) for strains 2) revealing differences in sensitivity among the two tested strains of D. magna. These results suggest that after carbaryl exposure, the AChE activity responses can be also used as a biomarker of susceptibility. Moreover, our results show that strain1 is less sensitive than strain 2 in terms of IC50-48 h of AChE activity. Comparing the EC50-48 h of standard ecotoxicity test and IC50-48 h of AChE inhibition, there is the same order of sensitivity with both strains.
本研究旨在调查西维因（氨基甲酸酯类杀虫剂）对甲壳纲枝角类大型溞两个品系（同一克隆A）乙酰胆碱酯酶活性的影响。将四种西维因浓度（0.4、0.9、1.8和3.7 µg L(-1)）与对照乙酰胆碱酯酶活性进行比较。我们的结果表明，在西维因暴露48小时后，无论考虑哪两个品系，所有处理均导致乙酰胆碱酯酶活性显著下降。然而，就最低观察效应浓度而言（品系1为0.4 µg L(-1)，品系2为0.9 µg L(-1)），出现了不同的反应，这揭示了两个受试大型溞品系之间的敏感性差异。这些结果表明，西维因暴露后，乙酰胆碱酯酶活性反应也可作为易感性生物标志物。此外，我们的结果表明，就乙酰胆碱酯酶活性的48小时半数抑制浓度而言，品系1比品系2敏感性低。比较标准生态毒性试验的48小时半数有效浓度和乙酰胆碱酯酶抑制的48小时半数抑制浓度，两个品系的敏感性顺序相同。
