Drážovská Monika, Šiviková Katarína, Holečková Beáta, Dianovský Ján, Galdíková Martina, Schwarzbacherová Viera
a Department of Biology and Genetics , University of Veterinary Medicine and Pharmacy in Košice , Košice , Slovak Republic.
J Environ Sci Health B. 2016 Nov;51(11):769-76. doi: 10.1080/03601234.2016.1198643. Epub 2016 Jul 18.
Potential genotoxic/cytotoxic effects of the epoxiconazole/fenpropimorph-based fungicide were investigated using single cell gel electrophoresis and cytogenetic assays: chromosomal aberrations, sister chromatid exchanges, micronuclei and fluorescence in situ hybridization in cultured bovine lymphocytes. No statistically significant elevations of DNA damage and increases in cytogenetic endpoints were seen. However, evident cytotoxic effect presented as a decrease in mitotic and proliferation indices were recorded after exposure of bovine lymphocytes to the fungicide for 24 and 48 h at concentrations ranging from 3 to 15 µg mL(-1) (P < 0.05, P < 0.01, P < 0.001). Similarly, for 24 h an inhibition in the cytokinesis block proliferation index (CBPI) was obtained after exposure to the fungicide at concentrations ranging from 1.5 to 15 µg mL(-1) (P < 0.01, P < 0.001) in each donor.
使用单细胞凝胶电泳和细胞遗传学检测方法，对基于环氧菌唑/粉唑醇的杀菌剂潜在的遗传毒性/细胞毒性作用进行了研究：在培养的牛淋巴细胞中检测染色体畸变、姐妹染色单体交换、微核和荧光原位杂交。未观察到DNA损伤的统计学显著升高以及细胞遗传学终点的增加。然而，在牛淋巴细胞暴露于浓度范围为3至15μg mL(-1)的杀菌剂24小时和48小时后，记录到明显的细胞毒性作用，表现为有丝分裂和增殖指数降低（P < 0.05，P < 0.01，P < 0.001）。同样，在每个供体中，在暴露于浓度范围为1.5至15μg mL(-1)的杀菌剂24小时后，获得了胞质分裂阻滞增殖指数（CBPI）的抑制（P < 0.01，P < 0.001）。
