Sisto Daneo L, Corvetti G, Filogamo G
Dipartimento di Anatomia e Fisiologia Umana, Università di Torino, Italy.
Biol Struct Morphog. 1989;2(1):25-30.
Immunohistochemical techniques with anti-desmin, anti-acetylcholine receptor and anti-fibronectin antisera and autohistoradiography were used to determine the dynamics of neuromuscular synaptogenesis. Fast twitching muscles were taken from chick embryos at 5 to 14 days of incubation. "Primitive eminences" at terminal arborizations of motor neurons were composed of Karnowsky positive, anti-desmin and anti-acetylcholine receptor positive cells which contained sites bound to alpha-bungarotoxin. These cells, characterized as myoblasts, fused with the myotubes during formation of neuromuscular junctions in the sites of contact with terminal arborizations of motor neurons. Their nuclei and cytoplasmic organelles become the nuclei and organelles in the soleplate.
使用抗结蛋白、抗乙酰胆碱受体和抗纤连蛋白抗血清的免疫组织化学技术以及放射自显影术来确定神经肌肉突触发生的动态过程。在孵化5至14天的鸡胚中获取快速抽搐肌肉。运动神经元终末分支处的“原始隆起”由卡诺夫斯基阳性、抗结蛋白和抗乙酰胆碱受体阳性细胞组成，这些细胞含有与α-银环蛇毒素结合的位点。这些被鉴定为成肌细胞的细胞，在与运动神经元终末分支接触的部位形成神经肌肉接头的过程中与肌管融合。它们的细胞核和细胞质细胞器成为终板中的细胞核和细胞器。
