• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

京尼平对顺铂在结肠癌细胞中细胞毒性的时间增强作用机制。

A Mechanism for the Temporal Potentiation of Genipin to the Cytotoxicity of Cisplatin in Colon Cancer Cells.

作者信息

Wang Ruihua, MoYung K C, Zhao Y J, Poon Karen

机构信息

1. Department of Gastroenterology, Shenzhen Hospital of Southern Medical University, Shenzhen, Guangdong,China 518100.

2. Program of Food Science and Technology, Division of Science and Technology, BNU-HKBU United International College, 28 Jinfeng Road, Tangjiawan, Zhuhai, Guangdong, China 519085.

出版信息

Int J Med Sci. 2016 Jun 29;13(7):507-16. doi: 10.7150/ijms.15449. eCollection 2016.

DOI:10.7150/ijms.15449
PMID:27429587
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4946121/
Abstract

OBJECTIVES

To investigate the potentiation effect of Genipin to Cisplatin induced cell senescence in HCT-116 colon cancer cells in vitro.

METHODS

Cell viability was estimated by Propidium iodide and Hoechst 3342, reactive oxygen species (ROS) with DHE, mitochondrial membrane potential (MMP) with JC-1 MMP assay Kit and electron current production with microbial fuel cells (MFC).

RESULTS

Genipin inhibited the UCP2 mediated anti-oxidative proton leak significantly promoted the Cisplatin induced ROS and subsequent cell death, which was similar to that of UCP2-siRNA. Cells treated with Cisplatin alone or combined with Genipin, ROS negatively, while MMP positively correlated with cell viability. Cisplatin induced ROS was significantly decreased by detouring electrons to MFC, or increased by Genipin combined treatment. Compensatory effects of UCP2 up-regulation with time against Genipin treatment were suggested. Shorter the Genipin treatment before Cisplatin better promoted the Cisplatin induced ROS and subsequent cell death.

CONCLUSION

The interaction of leaked electron with Cisplatin was important during ROS generation. Inhibition of UCP2-mediated proton leak with Genipin potentiated the cytotoxicity of Cisplatin. Owing to the compensatory effects against Genipin, shorter Genipin treatment before Cisplatin was recommended in order to achieve better potentiation effect.

摘要

目的

研究京尼平对顺铂诱导的HCT - 116结肠癌细胞体外细胞衰老的增强作用。

方法

用碘化丙啶和Hoechst 3342评估细胞活力，用二氢乙锭检测活性氧（ROS），用JC - 1线粒体膜电位检测试剂盒检测线粒体膜电位（MMP），用微生物燃料电池（MFC）检测电子电流产生。

结果

京尼平显著抑制UCP2介导的抗氧化质子泄漏，促进顺铂诱导的ROS产生及随后的细胞死亡，这与UCP2 - siRNA的作用相似。单独用顺铂或与京尼平联合处理的细胞，ROS与细胞活力呈负相关，而MMP与细胞活力呈正相关。通过将电子转移至MFC可显著降低顺铂诱导的ROS，而京尼平联合处理则使其增加。提示UCP2随时间上调对京尼平处理有代偿作用。顺铂处理前用京尼平处理的时间越短，越能更好地促进顺铂诱导的ROS产生及随后的细胞死亡。

结论

在ROS产生过程中，泄漏电子与顺铂的相互作用很重要。用京尼平抑制UCP2介导的质子泄漏可增强顺铂的细胞毒性。由于对京尼平有代偿作用，建议在顺铂处理前用京尼平处理的时间较短，以获得更好的增强效果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9563/4946121/b88b59bebc17/ijmsv13p0507g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9563/4946121/b32ceacb1690/ijmsv13p0507g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9563/4946121/6c89bb47165a/ijmsv13p0507g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9563/4946121/d78896266b9d/ijmsv13p0507g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9563/4946121/ebd7a0c84996/ijmsv13p0507g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9563/4946121/5c6066aed185/ijmsv13p0507g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9563/4946121/1c515c9060f9/ijmsv13p0507g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9563/4946121/b88b59bebc17/ijmsv13p0507g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9563/4946121/b32ceacb1690/ijmsv13p0507g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9563/4946121/6c89bb47165a/ijmsv13p0507g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9563/4946121/d78896266b9d/ijmsv13p0507g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9563/4946121/ebd7a0c84996/ijmsv13p0507g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9563/4946121/5c6066aed185/ijmsv13p0507g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9563/4946121/1c515c9060f9/ijmsv13p0507g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9563/4946121/b88b59bebc17/ijmsv13p0507g012.jpg

相似文献

1
A Mechanism for the Temporal Potentiation of Genipin to the Cytotoxicity of Cisplatin in Colon Cancer Cells.京尼平对顺铂在结肠癌细胞中细胞毒性的时间增强作用机制。
Int J Med Sci. 2016 Jun 29;13(7):507-16. doi: 10.7150/ijms.15449. eCollection 2016.
2
UCP2- and non-UCP2-mediated electric current in eukaryotic cells exhibits different properties.真核细胞中由解偶联蛋白2（UCP2）介导和非UCP2介导的电流表现出不同的特性。
Environ Sci Pollut Res Int. 2015 Dec;22(24):19618-31. doi: 10.1007/s11356-015-5155-6. Epub 2015 Aug 15.
3
Molecular mechanism of (18)F-FDG uptake reduction induced by genipin in T47D cancer cell and role of uncoupling protein-2 in cancer cell glucose metabolism.京尼平诱导T47D癌细胞中(18)F-FDG摄取减少的分子机制及解偶联蛋白-2在癌细胞葡萄糖代谢中的作用
Nucl Med Biol. 2016 Oct;43(10):587-92. doi: 10.1016/j.nucmedbio.2016.06.001. Epub 2016 Jun 7.
4
Effects of Mitochondrial Uncoupling Protein 2 Inhibition by Genipin in Human Cumulus Cells.京尼平对人卵丘细胞线粒体解偶联蛋白2的抑制作用
Biomed Res Int. 2015;2015:323246. doi: 10.1155/2015/323246. Epub 2015 Aug 19.
5
UCP2 inhibition sensitizes breast cancer cells to therapeutic agents by increasing oxidative stress.UCP2 抑制通过增加氧化应激使乳腺癌细胞对治疗药物敏感。
Free Radic Biol Med. 2015 Sep;86:67-77. doi: 10.1016/j.freeradbiomed.2015.04.032. Epub 2015 May 7.
6
Gallic acid induced apoptotic events in HCT-15 colon cancer cells.没食子酸诱导HCT - 15结肠癌细胞发生凋亡事件。
World J Gastroenterol. 2016 Apr 21;22(15):3952-61. doi: 10.3748/wjg.v22.i15.3952.
7
Promotion of p53 expression and reactive oxidative stress production is involved in zerumbone-induced cisplatin sensitization of non-small cell lung cancer cells.促进p53表达和活性氧化应激产生参与了莪术二酮诱导的非小细胞肺癌细胞对顺铂的敏感性。
Biochimie. 2014 Dec;107 Pt B:257-62. doi: 10.1016/j.biochi.2014.09.001. Epub 2014 Sep 16.
8
Genipin ameliorates age-related insulin resistance through inhibiting hepatic oxidative stress and mitochondrial dysfunction.栀子苷通过抑制肝氧化应激和线粒体功能障碍改善与年龄相关的胰岛素抵抗。
Exp Gerontol. 2013 Dec;48(12):1387-94. doi: 10.1016/j.exger.2013.09.001. Epub 2013 Sep 14.
9
Curcuminoid WZ26, a TrxR1 inhibitor, effectively inhibits colon cancer cell growth and enhances cisplatin-induced cell death through the induction of ROS.姜黄素 WZ26，一种 TrxR1 抑制剂，通过诱导 ROS 有效抑制结肠癌细胞生长，并增强顺铂诱导的细胞死亡。
Free Radic Biol Med. 2019 Sep;141:93-102. doi: 10.1016/j.freeradbiomed.2019.06.005. Epub 2019 Jun 6.
10
Enhancement of cisplatin-induced colon cancer cells apoptosis by shikonin, a natural inducer of ROS in vitro and in vivo.紫草素增强顺铂诱导的结肠癌细胞凋亡，紫草素是一种在体外和体内均可诱导活性氧（ROS）产生的天然物质。
Biochem Biophys Res Commun. 2016 Jan 22;469(4):1075-82. doi: 10.1016/j.bbrc.2015.12.100. Epub 2015 Dec 29.

引用本文的文献

1
Genipin-Simple but Significant Bioactive Iridoid for Therapeutical Application and Beyond: A Review.京尼平——一种用于治疗及其他用途的简单却重要的生物活性环烯醚萜：综述
Life (Basel). 2025 Jan 23;15(2):159. doi: 10.3390/life15020159.
2
Yiqi Chutan Formula Reverses Cisplatin-Induced Apoptosis and Ferroptosis of Skeletal Muscle by Alleviating Oxidative Stress.益气除痰方通过减轻氧化应激逆转顺铂诱导的骨骼肌细胞凋亡和铁死亡。
Integr Cancer Ther. 2023 Jan-Dec;22:15347354231172117. doi: 10.1177/15347354231172117.
3
Genipin, an Inhibitor of UCP2 as a Promising New Anticancer Agent: A Review of the Literature.

本文引用的文献

1
Mitochondrial Dysfunction and Disturbed Coherence: Gate to Cancer.线粒体功能障碍与相干性紊乱：通向癌症之门。
Pharmaceuticals (Basel). 2015 Sep 30;8(4):675-95. doi: 10.3390/ph8040675.
2
Induction of apoptosis by genipin inhibits cell proliferation in AGS human gastric cancer cells via Egr1/p21 signaling pathway.京尼平诱导的细胞凋亡通过Egr1/p21信号通路抑制AGS人胃癌细胞的增殖。
Bioorg Med Chem Lett. 2015 Oct 1;25(19):4191-6. doi: 10.1016/j.bmcl.2015.08.005. Epub 2015 Aug 7.
3
UCP2- and non-UCP2-mediated electric current in eukaryotic cells exhibits different properties.
栀子苷作为 UCP2 抑制剂：一种有前景的新型抗癌药物——文献综述。
Int J Mol Sci. 2022 May 18;23(10):5637. doi: 10.3390/ijms23105637.
4
Current research progress in the role of reactive oxygen species in esophageal adenocarcinoma.活性氧在食管腺癌中作用的当前研究进展
Transl Cancer Res. 2021 Mar;10(3):1568-1577. doi: 10.21037/tcr-19-1985.
5
Ethnopharmacology of Fruit Plants: A Literature Review on the Toxicological, Phytochemical, Cultural Aspects, and a Mechanistic Approach to the Pharmacological Effects of Four Widely Used Species.水果植物的民族药理学：四种广泛应用物种的毒理学、植物化学、文化方面以及药理学作用的机制研究的文献综述。
Molecules. 2020 Aug 26;25(17):3879. doi: 10.3390/molecules25173879.
6
Genipin induces mitochondrial dysfunction and apoptosis via downregulation of Stat3/mcl-1 pathway in gastric cancer.栀子苷通过下调 Stat3/mcl-1 通路诱导胃癌中线粒体功能障碍和细胞凋亡。
BMC Cancer. 2019 Jul 27;19(1):739. doi: 10.1186/s12885-019-5957-x.
7
Identification of driver genes and key pathways of prolactinoma predicts the therapeutic effect of genipin.鉴定催乳素瘤的驱动基因和关键通路可预测京尼平的治疗效果。
Mol Med Rep. 2019 Sep;20(3):2712-2724. doi: 10.3892/mmr.2019.10505. Epub 2019 Jul 18.
8
Plant-Derived Anticancer Agents: Lessons from the Pharmacology of Geniposide and Its Aglycone, Genipin.植物源抗癌剂：栀子苷及其苷元京尼平的药理学启示
Biomedicines. 2018 Mar 26;6(2):39. doi: 10.3390/biomedicines6020039.
9
Genipin inhibits the growth of human bladder cancer cells via inactivation of PI3K/Akt signaling.京尼平通过使PI3K/Akt信号失活来抑制人膀胱癌细胞的生长。
Oncol Lett. 2018 Feb;15(2):2619-2624. doi: 10.3892/ol.2017.7588. Epub 2017 Dec 11.
真核细胞中由解偶联蛋白2（UCP2）介导和非UCP2介导的电流表现出不同的特性。
Environ Sci Pollut Res Int. 2015 Dec;22(24):19618-31. doi: 10.1007/s11356-015-5155-6. Epub 2015 Aug 15.
4
Pharmacological inhibition of NADPH oxidase protects against cisplatin induced nephrotoxicity in mice by two step mechanism.抑制NADPH氧化酶的药理作用通过两步机制保护小鼠免受顺铂诱导的肾毒性。
Food Chem Toxicol. 2015 Sep;83:251-60. doi: 10.1016/j.fct.2015.05.007. Epub 2015 May 30.
5
UCP2 inhibition sensitizes breast cancer cells to therapeutic agents by increasing oxidative stress.UCP2 抑制通过增加氧化应激使乳腺癌细胞对治疗药物敏感。
Free Radic Biol Med. 2015 Sep;86:67-77. doi: 10.1016/j.freeradbiomed.2015.04.032. Epub 2015 May 7.
6
Lactate dehydrogenase inhibitors sensitize lymphoma cells to cisplatin without enhancing the drug effects on immortalized normal lymphocytes.乳酸脱氢酶抑制剂可使淋巴瘤细胞对顺铂敏感，而不会增强该药物对永生化正常淋巴细胞的作用。
Eur J Pharm Sci. 2015 Jul 10;74:95-102. doi: 10.1016/j.ejps.2015.04.022. Epub 2015 Apr 28.
7
Dasatinib enhances cisplatin sensitivity in human esophageal squamous cell carcinoma (ESCC) cells via suppression of PI3K/AKT and Stat3 pathways.达沙替尼通过抑制PI3K/AKT和Stat3信号通路增强人食管鳞状细胞癌（ESCC）细胞对顺铂的敏感性。
Arch Biochem Biophys. 2015 Jun 1;575:38-45. doi: 10.1016/j.abb.2014.11.008. Epub 2015 Apr 2.
8
Emodin augments cisplatin cytotoxicity in platinum-resistant ovarian cancer cells via ROS-dependent MRP1 downregulation.大黄素通过活性氧依赖性多药耐药相关蛋白1（MRP1）下调增强顺铂对铂耐药卵巢癌细胞的细胞毒性。
Biomed Res Int. 2014;2014:107671. doi: 10.1155/2014/107671. Epub 2014 Dec 14.
9
Reactive oxygen species generation is essential for cisplatin-induced accelerated senescence in hepatocellular carcinoma.活性氧生成对于顺铂诱导的肝细胞癌加速衰老至关重要。
Front Med. 2014 Jun;8(2):227-35. doi: 10.1007/s11684-014-0327-1. Epub 2014 Apr 22.
10
Ca(2+)-mediated regulation of VDAC1 expression levels is associated with cell death induction.钙离子介导的电压依赖性阴离子通道蛋白1（VDAC1）表达水平调控与细胞死亡诱导相关。
Biochim Biophys Acta. 2014 Oct;1843(10):2270-81. doi: 10.1016/j.bbamcr.2014.03.021. Epub 2014 Apr 1.