Sagha Mohsen, Najafzadeh Nowruz
Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
Methods Mol Biol. 2016;1453:161-72. doi: 10.1007/978-1-4939-3786-8_17.
Neural differentiation of hair-follicle-associated pluripotent (HAP) stem cells residing in the bulge area is a promising autologous source for stem cell therapy. In the present chapter, we describe the identification and enrichment of CD34(+) HAP stem cells by magnetic-activated cell sorting (MACS), and induce them to differentiate into neuronal and glial cells using defined neural-induction media. The different neural cell populations arising during in vitro differentiation from HAP stem cells are characterized by reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry assay.
位于毛囊隆突区的毛囊相关多能（HAP）干细胞的神经分化是干细胞治疗中一种很有前景的自体来源。在本章中，我们描述了通过磁珠分选法（MACS）鉴定和富集CD34(+) HAP干细胞，并使用特定的神经诱导培养基诱导它们分化为神经元和神经胶质细胞。通过逆转录聚合酶链反应（RT-PCR）和免疫细胞化学分析对体外分化过程中从HAP干细胞产生的不同神经细胞群体进行表征。
