Venturinil C G, Bruinsmann A, Oliveira C P, Contri R V, Pohlmann A R, Guterres S S
J Nanosci Nanotechnol. 2016 Feb;16(2):1310-20. doi: 10.1166/jnn.2016.11666.
An innovative nanocapsule formulation for parenteral administration using selected vegetable oils (mango, jojoba, pequi, oat, annatto, calendula, and chamomile) was developed that has the potential to encapsulate various drugs. The vegetable oil-loaded nanocapsules were prepared by interfacial deposition and compared with capric/caprylic triglyceride-loaded lipid core nanocapsules. The major objective was to investigate the effect of vegetable oils on particle size distribution and physical stability and to determine the hemolytic potential of the nanocapsules, considering their applicability for intravenous administration. Taking into account the importance of accurately determining particle size for the selected route of administration, different size characterization techniques were employed, such as Laser Diffraction, Dynamic Light Scattering, Multiple Light Scattering, Nanoparticle Tracking Analysis, and Transmission Electronic Microscopy. Laser diffraction studies indicated that the mean particle size of all nanocapsules was below 300 nm. For smaller particles, the laser diffraction and multiple light scattering data were in agreement (D[3,2]-130 nm). Dynamic light scattering and nanoparticle tracking analysis, two powerful techniques that complement each other, exhibited size values between 180 and 259 nm for all nanoparticles. Stability studies demonstrated a tendency of particle creaming for jojoba-nanocapsules and sedimentation for the other nanoparticles; however, no size variation occurred over 30 days. The hemolysis test proved the hemocompatibility of all nanosystems, irrespective of the type of oil. Although all developed nanocapsules presented the potential for parenteral administration, jojoba oil-loaded nanocapsules were selected as the most promising nanoformulation due to their low average size and high particle size homogeneity.
开发了一种创新的纳米胶囊制剂，用于肠胃外给药，该制剂使用了选定的植物油（芒果油、荷荷巴油、巴西腰果油、燕麦油、胭脂树籽油、金盏花油和洋甘菊油），有潜力包裹各种药物。通过界面沉积法制备了负载植物油的纳米胶囊，并与负载癸酸/辛酸甘油三酯的脂质核纳米胶囊进行了比较。主要目的是研究植物油对粒径分布和物理稳定性的影响，并确定纳米胶囊的溶血潜力，同时考虑其静脉给药的适用性。考虑到准确测定所选给药途径的粒径的重要性，采用了不同的粒径表征技术，如激光衍射、动态光散射、多重光散射、纳米颗粒跟踪分析和透射电子显微镜。激光衍射研究表明，所有纳米胶囊的平均粒径均低于300nm。对于较小的颗粒，激光衍射和多重光散射数据一致（D[3,2]-130nm）。动态光散射和纳米颗粒跟踪分析这两种相互补充的强大技术显示，所有纳米颗粒的粒径值在180至259nm之间。稳定性研究表明，荷荷巴纳米胶囊有颗粒上浮趋势，其他纳米颗粒有沉降趋势；然而，30天内粒径没有变化。溶血试验证明了所有纳米系统的血液相容性，与油的类型无关。尽管所有开发的纳米胶囊都具有肠胃外给药的潜力，但由于其平均粒径低且粒径均匀性高，负载荷荷巴油的纳米胶囊被选为最有前景的纳米制剂。
