Park Hyun Jung, Ahn Joo-Myung, Park Ra-Mi, Lee Sang-Hee, Sekhon Simranjeet Singh, Kim Sang Yong, Wee Ji-Hyang, Kim Yang-Hoon, Min Jiho
J Nanosci Nanotechnol. 2016 Feb;16(2):1445-9. doi: 10.1166/jnn.2016.10757.
In this study, we report an oral drug delivery system without any additional process using pH-sensitive biopolymer, alginate, and alginate oligosaccharide with lysozyme as a model drug. These biopolymers exhibited pH-sensitive characteristics such as shrinking at acidic pH and eroding with dissolution at alkaline pH. The incorporation of lysozyme and biopolymers was performed an artificial intestinal juice (pH 6.8). The immobilization efficiency and lysozyme stability in gastric juice (pH 1.2) has been tested by E coil antimicrobial activity. The lysozyme without biopolymer immobilization lost approximately 80-90% of antimicrobial activity than that of pure lysozyme. However, the pH-sensitive biopolymer-controlled lysozyme maintained similar antimicrobial activity compared to that of pure lysozyme (50-90% of cell mortality). Therefore, this simple, easy, and rapid system can be effectively and practically applied for pathogen treatment for in vivo oral drug delivery.
在本研究中，我们报道了一种无需任何额外处理的口服给药系统，该系统使用对pH敏感的生物聚合物海藻酸盐和海藻酸寡糖，并以溶菌酶作为模型药物。这些生物聚合物表现出pH敏感特性，如在酸性pH下收缩，在碱性pH下随着溶解而侵蚀。溶菌酶与生物聚合物的结合在人工肠液（pH 6.8）中进行。通过大肠杆菌抗菌活性测试了溶菌酶在胃液（pH 1.2）中的固定效率和稳定性。未固定生物聚合物的溶菌酶比纯溶菌酶失去了约80-90%的抗菌活性。然而，与纯溶菌酶相比，对pH敏感的生物聚合物控制的溶菌酶保持了相似的抗菌活性（细胞死亡率为50-90%）。因此，这种简单、易行且快速的系统可有效且实际地应用于体内口服给药的病原体治疗。
