Cernák A, Kalafut F, Kusenda J, Novotná L
Cesk Oftalmol. 1989 May;45(3):145-52.
Monoclonal antibodies (MA) were obtained by fusion of a myeloma line from mice with spleen cells of mice previously immunized by live human cells of uveal melanoma of an epithelioid type (UMEL-H). From the cell fusion hybridomas were obtained which produced antibodies against immunized uveal melanoma cells. The binding capacity of MA was assessed by the sandwich radio-immuno (RIA) test using indirect immunofluorescence. Extensive specific tests revealed that MA are strongly bound to uveal melanoma, the cells of which were used to immunize the mice, but also fresh allogenic uveal melanoma cells UMEL-K which were also of the epithelioid type. A smaller binding activity was observed with the cell line of the uveal melanoma VUP-1 which was started 16 years ago and is formed by 92% by epithelioid cells. A smaller binding activity of MA was observed with the line of skin melanoma cells (HMB-2, B-HM8). The bond of MA with carcinomatous cells, fibroblasts, uveal and retinal cells from eyes of healthy humans was negative. The preliminary investigations indicate an antigenic homogeneity of tumour antigens of melanoma cells of the same histological type and the antigenic variability of uveal and skin melanomas.
通过将源自小鼠的骨髓瘤细胞系与先前用上皮样型葡萄膜黑色素瘤（UMEL-H）的活人类细胞免疫的小鼠脾细胞融合，获得了单克隆抗体（MA）。从细胞融合中获得了杂交瘤，其产生针对免疫的葡萄膜黑色素瘤细胞的抗体。使用间接免疫荧光通过夹心放射免疫（RIA）试验评估MA的结合能力。广泛的特异性试验表明，MA与用于免疫小鼠的葡萄膜黑色素瘤细胞紧密结合，而且还与同样为上皮样型的新鲜同种异体葡萄膜黑色素瘤细胞UMEL-K紧密结合。对于16年前建立且92%由上皮样细胞构成的葡萄膜黑色素瘤VUP-1细胞系，观察到较小的结合活性。对于皮肤黑色素瘤细胞系（HMB-2、B-HM8），观察到MA具有较小的结合活性。MA与来自健康人眼睛的癌细胞、成纤维细胞、葡萄膜和视网膜细胞的结合呈阴性。初步研究表明，相同组织学类型的黑色素瘤细胞的肿瘤抗原具有抗原同质性，而葡萄膜和皮肤黑色素瘤具有抗原变异性。
