Damato B E, Campbell A M, McGuire B J, Lee W R, Foulds W S
Invest Ophthalmol Vis Sci. 1986 Sep;27(9):1362-7.
Five rat monoclonal antibodies to human uveal melanoma were produced using primary tumor tissue for immunization and screening. These monoclonal antibodies were tested by enzyme-linked immunosorbent assay against 12 uveal melanomas, one breast carcinoma, and peripheral blood lymphocytes from five healthy volunteers. One monoclonal antibody, 4A3, reacted against all the melanomas and none of the controls. On immunoblotting, this monoclonal antibody identified a protein doublet in the molecular weight range 55,000. With these monoclonal antibodies, it was possible to demonstrate significant variation in the antigenic profiles of the uveal melanomas which was not present in the controls.
利用原发性肿瘤组织进行免疫和筛选，制备了5种针对人葡萄膜黑色素瘤的大鼠单克隆抗体。通过酶联免疫吸附测定法，用这些单克隆抗体检测了12例葡萄膜黑色素瘤、1例乳腺癌以及5名健康志愿者的外周血淋巴细胞。其中一种单克隆抗体4A3与所有黑色素瘤发生反应，而与所有对照均无反应。在免疫印迹分析中，该单克隆抗体识别出分子量在55,000范围内的蛋白双峰。利用这些单克隆抗体，可以证明葡萄膜黑色素瘤的抗原谱存在显著差异，而对照中不存在这种差异。
