Huang L, Yin Z J, Feng Y F, Zhang X D, Wu T, Ding Y Y, Ye P F, Fu K, Zhang M Q
Anhui Provincial Laboratory of Local Animal Genetic Resource Conservation and Bio-Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, 230036, P. R. China.
Anim Genet. 2016 Oct;47(5):543-51. doi: 10.1111/age.12452. Epub 2016 Jul 20.
Litter size affects profitability in the swine industry. Mammalian ovaries play important roles during reproduction, including ovulation and hormone secretion, which are tightly regulated by specific microRNAs (miRNAs). In this study, we investigated the effects of specific miRNAs on porcine litter size. We compared the ovarian miRNAs of Yorkshire pigs with high (YH) and low (YL) litter sizes using Solexa sequencing technology. We identified 327 and 320 miRNAs in the ovaries of YH and YL pigs respectively. A total of 297 miRNAs were co-expressed; 30 and 23 miRNAs respectively were specifically expressed in the two libraries. A total of 83 novel miRNAs were predicted; 37 specific miRNAs were obtained, of which 21 miRNAs were upregulated and 16 miRNAs were downregulated in YH compared with YL. Additionally, 19 628 and 19 250 target genes were predicted in the two libraries respectively. The results revealed that specific miRNAs (i.e., miR-224, miR-99a, let-7c, miR-181c, miR-214 and miR-21) may affect porcine litter size. The results of this study will help in gaining understanding of the role of miRNAs in porcine litter size regulation.
产仔数影响养猪业的盈利能力。哺乳动物卵巢在繁殖过程中发挥着重要作用，包括排卵和激素分泌，这些过程受到特定微小RNA（miRNA）的严格调控。在本研究中，我们调查了特定miRNA对猪产仔数的影响。我们使用Solexa测序技术比较了高（YH）产仔数和低（YL）产仔数的约克夏猪的卵巢miRNA。我们分别在YH和YL猪的卵巢中鉴定出327个和320个miRNA。共有297个miRNA共表达；两个文库中分别有30个和23个miRNA特异性表达。共预测出83个新的miRNA；获得了37个特异性miRNA，其中与YL相比，YH中有21个miRNA上调，16个miRNA下调。此外，两个文库中分别预测出19628个和19250个靶基因。结果表明，特定的miRNA（即miR-224、miR-99a、let-7c、miR-181c、miR-214和miR-21）可能影响猪的产仔数。本研究结果将有助于了解miRNA在猪产仔数调控中的作用。
