温度对检测白纹伊蚊中杀螟硫磷抗性的酶测定的影响。
Effect of temperature on an enzyme assay to detect fenitrothion resistance in Anopheles albimanus mosquitos.
作者信息
Beach R F, Brogdon W G, Castañaza L A, Cordón-Rosales C, Calderón M
出版信息
Bull World Health Organ. 1989;67(2):203-8.
A laboratory strain of Anopheles albimanus Wiedemann of known fenitrothion resistance was used in the field to compare the results of the WHO test for determining fenitrothion resistance in mosquitos with those of an enzyme microplate assay. The level of resistance obtained with the enzyme assay increased with the ambient temperature, and in order to compensate for this temperature effect, the incubation time was reduced. With the adjusted incubation times, the results for the microassay from 23 degrees C to 32 degrees C were the same as those found with the WHO test. The fenitrothion resistance of a field population of A. albimanus mosquitos determined between 27 degrees C and 31 degrees C using the adjusted enzyme microassay or the WHO test did not differ in a statistically significant way.
使用对杀螟硫磷具有已知抗性的白纹伊蚊实验室品系在野外比较世界卫生组织（WHO）用于测定蚊子对杀螟硫磷抗性的试验结果与酶联免疫吸附测定的结果。酶测定所获得的抗性水平随环境温度升高而增加，为补偿这种温度效应，孵育时间被缩短。通过调整孵育时间，在23摄氏度至32摄氏度下微量测定的结果与WHO试验的结果相同。使用调整后的酶联免疫吸附测定法或WHO试验在27摄氏度至31摄氏度之间测定的野外白纹伊蚊种群对杀螟硫磷的抗性在统计学上没有显著差异。
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