Brogdon W G, Hobbs J H, St Jean Y, Jacques J R, Charles L B
Division of Parasitic Diseases, Centers for Disease Control, Atlanta, GA 30333.
J Am Mosq Control Assoc. 1988 Jun;4(2):152-8.
Reduced fenitrothion susceptibility in Haitian Anopheles albimanus is documented using time/mortality measurements from otherwise standard World Health Organization (WHO) bioassays. Survival beyond a time threshold in bioassays is shown to be highly correlated with elevated non-specific esterase levels. A shift in resistance incidence from less than 20 to over 60% in a six-month period is documented using both the bioassay and microassay procedures, showing the potential of microplate assay methods in early detection of resistance. Conventional 24-hour reading of WHO bioassay data failed to detect resistance until the level reached 60%. Resistance appeared to be focal, with an increase in intensity coinciding with a fenitrothion spray cycle for malaria control.
通过世界卫生组织（WHO）标准生物测定中的时间/死亡率测量，记录了海地白纹伊蚊对杀螟硫磷的敏感性降低情况。生物测定中超过时间阈值的存活情况显示与非特异性酯酶水平升高高度相关。使用生物测定和微量测定程序记录了六个月内抗性发生率从不到20%转变为超过60%的情况，显示了微孔板测定方法在早期检测抗性方面的潜力。常规对WHO生物测定数据进行24小时读数，直到抗性水平达到60%才检测到抗性。抗性似乎是局部性的，强度增加与用于疟疾控制的杀螟硫磷喷洒周期一致。
