Wu Chuan-Wen, Zhang Xiu-Feng, Liu Wei, Wang Hong-Li, Hao Xiao-Hui, Guo Zhi-Yi, Guo Ling-Li, Liu He-Liang
Public Health School, North China University of Science and Technology, Tangshan 063000, China.E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2016 Jun 20;36(7):1004-7.
To investigate the relationship between polymorphisms of surfactant protein D (rs3088308 and rs721917) and the susceptibility to silicosis.
This case-control study included 125 silicosis patients and 125 individuals exposed to industrial dust but without silicosis (control group), who were strictly matched with the case group for age, gender, work type and cumulative length of dust exposure. The rs3088308 and rs721917 polymorphisms of surfactant protein-D were detected in all the participants using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
The frequencies of T/T, T/A and A/A genotypes of surfactant protein-D rs3088308 locus were 22.2%, 71.2% and 5.6% in the case group, significantly different from the frequencies of 17.6%, 58.4% and 24.0% in the control group, respectively (P<0.05). The frequencies of C/C, C/T and T/T genotypes of rs721917 locus were 17.6%, 56.8% and 25.6% in the case group, similar to the frequencies of 15.2%, 60.0% and 24.8% in the control group, respectively (P>0.05).
Surfactant protein-D rs3088308 polymorphism is significantly associated with silicosis, and the T allele may be a risk factor for silicosis in individuals exposed to industrial dust.
探讨表面活性蛋白D基因多态性（rs3088308和rs721917）与矽肺易感性之间的关系。
本病例对照研究纳入125例矽肺患者和125名接触工业粉尘但未患矽肺的个体（对照组），对照组在年龄、性别、工作类型和累积粉尘接触时长方面与病例组进行严格匹配。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测所有参与者表面活性蛋白D的rs3088308和rs721917基因多态性。
病例组中表面活性蛋白D rs3088308位点的T/T、T/A和A/A基因型频率分别为22.2%、71.2%和5.6%，与对照组中17.6%、58.4%和24.0%的频率相比，差异有统计学意义（P<0.05）。rs721917位点的C/C、C/T和T/T基因型频率在病例组中分别为17.6%、56.8%和25.6%，与对照组中15.2%、60.0%和24.8%的频率相似（P>0.05）。
表面活性蛋白D rs3088308基因多态性与矽肺显著相关，T等位基因可能是接触工业粉尘个体患矽肺的危险因素。
