Department of Occupational and Environmental Health, School of Public Health, Hebei United University, Tangshan 063000, Hebei, China.
Biomed Environ Sci. 2011 Dec;24(6):678-82. doi: 10.3967/0895-3988.2011.06.013.
To explore the relationship between polymorphisms of interleukin-4 (IL-4) gene (-33, +45, intron3, +429, +448) and the susceptibility of silicosis.
A case-control study was carried out. 101 silicosis patients were selected as cases. As strictly matching, 121 of non silicosis workers were selected as the controls. The polymophisms of IL-4 (five locus) were detected by the method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques.
The GA genotype in the IL-4+429 locus and the CC genotype in the IL-4+448 locus were found. The frequencies of AA, GG and AG of IL-4+45 locus in the cases were 55.4%, 10.9%, and 33.7% and in the controls were 62.0%, 11.6%, and 26.4%. The differences between cases and controls were not significant. The frequencies of B1B1, B2B2, and B1B2 of intron3 VNTR locus in the cases were 73.3%, 1.0%, and 25.7% and in the controls were 68.6%, 1.7%, and 29.8%. The differences were not significant. The frequencies of TT, CC, and CT in -33 locus in the cases were 55.4%, 11.9%, and 32.7% and in the controls were 69.4%, 4.1%, and 26.4%. The differences were significant (P=0.034).
The relationship between genetic polymorphism of IL-4-33 site and silicosis has been found and -33TT is a protective genotype for silicosis.
探讨白细胞介素 4（IL-4）基因（-33、+45、内含子 3、+429、+448）多态性与矽肺易感性的关系。
采用病例对照研究，选择 101 例矽肺患者为病例，严格配对选择 121 例非矽肺工人为对照，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测 IL-4（5 个位点）多态性。
发现 IL-4+429 位点的 GA 基因型和 IL-4+448 位点的 CC 基因型，IL-4+45 位点病例组 AA、GG 和 AG 基因型频率分别为 55.4%、10.9%和 33.7%，对照组分别为 62.0%、11.6%和 26.4%，两组间差异无统计学意义。内含子 3VNTR 位点病例组 B1B1、B2B2 和 B1B2 基因型频率分别为 73.3%、1.0%和 25.7%，对照组分别为 68.6%、1.7%和 29.8%，两组间差异无统计学意义。-33 位点病例组 TT、CC 和 CT 基因型频率分别为 55.4%、11.9%和 32.7%，对照组分别为 69.4%、4.1%和 26.4%，两组间差异有统计学意义（P=0.034）。
发现白细胞介素 4-33 位点遗传多态性与矽肺的关系，-33TT 是矽肺的保护基因型。
