Howlett C R, McCartney E, Ching W
School of Pathology, University of New South Wales, Kensington, Australia.
Clin Orthop Relat Res. 1989 Jul(244):293-304.
This article presents a morphologic assessment of the effect of silicon nitride ceramic (Si3N4) on rabbit marrow stromal cells and their differentiation when grown in vitro and in vivo. In vitro marrow stromal cells (MSC) attached initially to upper portions of ceramic discs. However, at four weeks, cells only attached to disc edges. Fresh marrow or first passage MSC, inoculated into diffusion chambers with and without Si3N4, formed cartilage, bone, and fibrous tissue after being implanted intraperitoneally for five weeks. Tissue differentiated adjacent to Si3N4 but not within the pores. In contrast, Si3N4 implants inserted into femoral marrow cavities were surrounded initially by woven bone and within three months by mature bone that had permeated implants with a pore size of 255 +/- 64 microns. Plugs having a pore diameter of 170 +/- 45 microns mainly contained vascularized fibrous tissue with occasional foci of osteoid or bone in the peripheral pores. In a pilot experiment, three femoral segmental Si3N4 endoprostheses were implanted in three adult rabbits, and the osseous reactions were monitored during their natural life. Each implant was enclosed by a stable cuff of bone within four months of implantation and remained unchanged during the rest of the animal's life. Autopsies confirmed these roentgenographic observations, and tissue appositional to each prosthesis was morphologically normal. Si3N4 has the potential of an important ceramic for use in osseous reconstruction.
本文介绍了氮化硅陶瓷（Si3N4）对兔骨髓基质细胞的影响及其在体内外生长时的分化情况的形态学评估。体外实验中，骨髓基质细胞（MSC）最初附着在陶瓷盘的上部。然而，四周后，细胞仅附着在盘边缘。将新鲜骨髓或第一代传代MSC接种到含或不含Si3N4的扩散室中，腹腔内植入五周后形成软骨、骨和纤维组织。组织在Si3N4附近分化，但不在孔隙内。相比之下，插入股骨髓腔的Si3N4植入物最初被编织骨包围，三个月内被成熟骨包围，成熟骨已渗透到孔径为255±64微米的植入物中。孔径为170±45微米的塞子主要含有血管化纤维组织，外周孔隙偶尔有类骨质或骨灶。在一项初步实验中，将三个股段Si3N4假体植入三只成年兔体内，并在其自然寿命期间监测骨反应。每个植入物在植入后四个月内被一层稳定的骨袖包围，在动物的余生中保持不变。尸检证实了这些X线观察结果，每个假体周围的组织形态正常。Si3N4有潜力成为骨重建中一种重要的陶瓷材料。
