Li Dong, Gao Xiuzhu, Gu Jingmin, Tian Yuan, Liu Yaqing, Jin Zheng, Yan Dongmei, Chen Ya-Guang, Zhu Xun
Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
Department of Hepatology, First Hospital of Jilin University, Changchun 130021, China.
Bioinorg Chem Appl. 2016;2016:3239494. doi: 10.1155/2016/3239494. Epub 2016 Jun 29.
Objective. Asthma is a chronic inflammatory disorder. Despite extensive researches into the treatment and management of it, current treatments and management strategies are still limited. The search for a novel approach to its treatments is urgently needed. Researches on the potential medical use of polyoxometalates (POMs) have already shown it has antiviral and antitumor bioactivities. But the effects of POM in immune systems are still largely unknown. Methods. In order to investigate the role of POM in the asthmatic disease, we used OVA-induced asthma murine model and observed the pathological changes between mice that received three different Ti-substituted POMs (0.3 μg per mouse per dose) when challenged with OVA. We also measured the type 2 cytokine expressions to reveal the potential mechanism. Results and Conclusions. Our results showed that two Ti-substituted POMs, K5H2[FeW11TiO40]·17H2O and K5H[H2ZnW11TiO40]·35H2O, could reduce OVA-induced lung inflammation, serum IgE level (around 2000 ng/mL to less than 1000 ng/mL), leukocytes infiltration in the lung, and cytokines levels (including IL-4, IL-5, IL-13, and TNF-α) but Ti-centered POM K4[TiW12O40]·10H2O did not. Thus, Ti-substituted POMs may have pharmaceutical values especially in treatments for asthmatic diseases.
目的。哮喘是一种慢性炎症性疾病。尽管对其治疗和管理进行了广泛研究，但目前的治疗方法和管理策略仍然有限。迫切需要寻找一种新的治疗方法。对多金属氧酸盐（POMs）潜在医学用途的研究已经表明其具有抗病毒和抗肿瘤生物活性。但POM在免疫系统中的作用仍 largely unknown。方法。为了研究POM在哮喘疾病中的作用，我们使用卵清蛋白（OVA）诱导的哮喘小鼠模型，观察了接受三种不同的钛取代POMs（每只小鼠每剂量0.3μg）的小鼠在受到OVA攻击时的病理变化。我们还测量了2型细胞因子的表达以揭示潜在机制。结果与结论。我们的结果表明，两种钛取代的POMs，K5H2[FeW11TiO40]·17H2O和K5H[H2ZnW11TiO40]·35H2O，可以减轻OVA诱导的肺部炎症、血清IgE水平（从约2000ng/mL降至低于1000ng/mL）、肺部白细胞浸润以及细胞因子水平（包括IL-4、IL-5、IL-13和TNF-α），但以钛为中心的POM K4[TiW12O40]·10H2O则没有。因此，钛取代的POMs可能具有药用价值，尤其是在哮喘疾病的治疗中。
