Luo Landi, Zhang Pin, Ou Xiaokun, Geng Yupeng
Institute of Ecology and Geobotany, School of Ecology and Environmental Sciences, Yunnan University, Kunming 650091, People's Republic of China.
Appl Plant Sci. 2016 Jul 12;4(7). doi: 10.3732/apps.1600011. eCollection 2016 Jul.
Tithonia diversifolia (Asteraceae) is an invasive plant species that can outcompete natives and thus poses a great threat to biodiversity in introduced areas. Here, expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed and characterized.
Sixteen polymorphic microsatellite loci were isolated from T. diversifolia using transcriptome sequencing and bioinformatic screening. The number of alleles per locus varied from two to four alleles in 48 individuals from three populations. Most of these primers also amplified in T. rotundifolia and some even in Parthenium hysterophorus.
These markers are useful for investigating the genetic structure and evolutionary process of T. diversifolia, which may provide important information for better management.
肿柄菊（菊科）是一种入侵植物物种，它能够胜过本地植物，因此对引入地区的生物多样性构成巨大威胁。在此，开发并鉴定了表达序列标签-简单序列重复（EST-SSR）标记。
利用转录组测序和生物信息学筛选，从肿柄菊中分离出16个多态性微卫星位点。在来自三个种群的48个个体中，每个位点的等位基因数量从两个到四个不等。这些引物中的大多数也能在圆叶肿柄菊中扩增，甚至有些还能在银胶菊中扩增。
这些标记对于研究肿柄菊的遗传结构和进化过程很有用，这可能为更好的管理提供重要信息。
