唇形科某变种EST-SSR标记的开发与特征分析
Development and characterization of EST-SSR markers for var. (Lamiaceae).
作者信息
Liu Lele, Wang Jingwen, Yin Meiqi, Guo Xiao, Cai Yunfei, Du Ning, Yu Xiaona, Guo Weihua
机构信息
Institute of Ecology and Biodiversity School of Life Sciences Shandong University Qingdao 266237 People's Republic of China.
College of Landscape Architecture and Forestry Qingdao Agricultural University Qingdao 266109 People's Republic of China.
出版信息
Appl Plant Sci. 2019 Jan 7;7(1):e01209. doi: 10.1002/aps3.1209. eCollection 2019 Jan.
PREMISE OF THE STUDY
var. (Lamiaceae) is a dominant shrub in the warm temperate zone of northern China. Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed to investigate its genetic diversity and structure.
METHODS AND RESULTS
We detected 12,075 SSRs in var. using transcriptome sequencing. Primer pairs for 100 SSR loci were designed and amplified in three populations of var. . Sixty loci were amplified, of which 14 were polymorphic. The number of alleles per locus ranged from two to 15, and levels of observed and expected heterozygosity ranged from 0.241 to 0.828 and from 0.426 to 0.873, respectively. All primer pairs amplified PCR products from but only four of them amplified products from .
CONCLUSIONS
The identified EST-SSR markers will be useful for future molecular and reproductive ecology studies of var. and .
研究前提
荆条（马鞭草科）是中国北方暖温带的优势灌木。开发了表达序列标签-简单序列重复（EST-SSR）标记以研究其遗传多样性和结构。
方法与结果
我们通过转录组测序在荆条中检测到12,075个SSR。设计了100个SSR位点的引物对，并在三个荆条种群中进行扩增。扩增出60个位点，其中14个具有多态性。每个位点的等位基因数从2到15不等，观察到的杂合度和预期杂合度水平分别从0.241到0.828和从0.426到0.873。所有引物对均从荆条中扩增出PCR产物，但其中只有四个从[未提及的物种]中扩增出产物。
结论
鉴定出的EST-SSR标记将有助于未来对荆条和[未提及的物种]进行分子和繁殖生态学研究。
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