Navid Muhammad Tariq, Li Yingying, Zhou Ming, Cui Min, Fu Zhen F, Tang Lijun, Zhao Ling
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
Arch Virol. 2016 Oct;161(10):2863-70. doi: 10.1007/s00705-016-2967-8. Epub 2016 Jul 20.
Two recombinant rabies viruses overexpressing their glycoprotein (G) were compared in this study, with the overexpressed G inserted between P and M genes (named LBNSE-PM-G), and between the G and L genes (named LBNSE-GL-G), respectively. LBNSE-PM-G produced more G protein and induced stronger apoptosis than LBNSE-GL-G in infected cells, while the amount of virion-incorporated G in LBNSE-PM-G was less than in LBNSE-GL-G. Mice immunized with inactivated LBNSE-PM-G produced lower titers of virus-neutralizing antibody, and this recombinant conferred worse protection than LBNSE-GL-G. Our results suggest that over expressed G gene inserted between G and L, but not between P and M, enhanced the immunogenicity when used as an inactivated rabies vaccine.
在本研究中，对两种过表达其糖蛋白（G）的重组狂犬病病毒进行了比较，过表达的G分别插入到P基因和M基因之间（命名为LBNSE-PM-G）以及G基因和L基因之间（命名为LBNSE-GL-G）。在感染细胞中，LBNSE-PM-G比LBNSE-GL-G产生更多的G蛋白并诱导更强的细胞凋亡，而LBNSE-PM-G中病毒体结合的G量少于LBNSE-GL-G。用灭活的LBNSE-PM-G免疫的小鼠产生较低滴度的病毒中和抗体，并且这种重组体提供的保护比LBNSE-GL-G更差。我们的结果表明，插入到G基因和L基因之间而非P基因和M基因之间的过表达G基因，在用作灭活狂犬病疫苗时增强了免疫原性。
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