Borneleit P, Blechschmidt B, Kleber H P
Sektion Biowissenschaften, Karl-Marx-Universität, Leipzig.
Electrophoresis. 1989 Apr;10(4):234-7. doi: 10.1002/elps.1150100403.
R-Form lipopolysaccharides of Acinetobacter calcoaceticus could be incorporated into polyacrylamide gels in an immobile form by adding it directly to the acrylamide-N,N'-methylenebisacrylamide polymerization mixture. The separation of A. calcoaceticus 69 V outer membrane proteins in these affinity gels demonstrated a specific interaction with the lipopolysaccharide ligand for one of the proteins. This protein is heat-modifiable and has an Mr of about 18,000. By incorporation of varying concentrations of lipopolysaccharide, a dissociation constant of the protein-lipopolysaccharide complex of 0.5 mM could be determined. In comparison, for another A. calcoaceticus strain, CCM 5593, a higher dissociation constant (1.0 mM)--indicative of lower affinity--was obtained.
通过将醋酸钙不动杆菌的R型脂多糖直接添加到丙烯酰胺-N，N'-亚甲基双丙烯酰胺聚合混合物中，它可以以固定形式掺入聚丙烯酰胺凝胶中。在这些亲和凝胶中对醋酸钙不动杆菌69 V外膜蛋白的分离表明，其中一种蛋白与脂多糖配体存在特异性相互作用。这种蛋白可被热修饰，分子量约为18,000。通过掺入不同浓度的脂多糖，可以确定蛋白-脂多糖复合物的解离常数为0.5 mM。相比之下，对于另一株醋酸钙不动杆菌CCM 5593，获得了更高的解离常数（1.0 mM），这表明其亲和力较低。
