Borneleit P, Hermsdorf T, Claus R, Walther P, Kleber H P
Sektion Biowissenschaften, Karl-Marx-Universität, Leipzig, GDR.
J Gen Microbiol. 1988 Jul;134(7):1983-92. doi: 10.1099/00221287-134-7-1983.
Lipopolysaccharide-rich vesicles were released from Acinetobacter calcoaceticus 69V during growth on hexadecane. Vesicle formation occurred over the whole surface of the cell as demonstrated by scanning electron microscopy. In contrast, the surface of acetate-grown cells, for which little lipopolysaccharide was found in the growth medium, appeared smooth. The overall chemical composition as well as the protein and phospholipid composition of the outer membranes of both cell types was very similar. In the vesicles all outer membrane proteins were found with the exception of an Mr 10,000 polypeptide corresponding to Braun's lipoprotein. Compared with the outer membrane, the vesicles contained more phosphatidylethanolamine. Hexadecane-grown cells were susceptible to exogenously added phospholipase. Nevertheless the barrier function towards lysozyme was retained.
在十六烷上生长期间，乙酸钙不动杆菌69V释放出富含脂多糖的囊泡。扫描电子显微镜显示，囊泡在细胞的整个表面形成。相比之下，在生长培养基中未发现脂多糖的乙酸盐培养细胞表面看起来很光滑。两种细胞类型的外膜的总体化学成分以及蛋白质和磷脂组成非常相似。在囊泡中，除了对应于布劳恩脂蛋白的10,000道尔顿多肽外，发现了所有外膜蛋白。与外膜相比，囊泡含有更多的磷脂酰乙醇胺。十六烷生长的细胞对外源添加的磷脂酶敏感。然而，对溶菌酶的屏障功能得以保留。
