Tovey E R, Ford S A, Baldo B A
Kolling Institute of Medical Research, Royal North Shore Hospital, St. Leonards, NSW, Australia.
Electrophoresis. 1989 Apr;10(4):243-9. doi: 10.1002/elps.1150100405.
The effect of blocking nitrocellulose membranes with the nonionic detergent Tween 20 on the detection, by protein blotting, of IgE-binding to house dust mite Dermatophagoides pteronyssinus allergens has been investigated. Tween blocking led to enhanced immunodetection of allergens despite removal of proteins from the membrane when compared to protein blocking agents which did not displace transferred components. The enhancement varied with the different mite components and, for one in particular, antigen Der p II, an increase of more than 100-fold in IgE antibody binding occurred despite a concurrent loss of more than 90% of Der p II from the membrane. Both the enhancement of binding and loss of components from the membrane were dependent upon the time course of blocking and the concentration of Tween used.
研究了用非离子去污剂吐温20封闭硝酸纤维素膜对通过蛋白质印迹法检测免疫球蛋白E（IgE）与屋尘螨过敏原结合的影响。与未置换转移成分的蛋白质封闭剂相比，吐温封闭导致过敏原免疫检测增强，尽管膜上的蛋白质被去除。增强效果因螨的不同成分而异，特别是对于一种抗原Der p II，尽管膜上同时损失了超过90%的Der p II，但IgE抗体结合增加了100倍以上。结合的增强和膜上成分的损失都取决于封闭的时间进程和所用吐温的浓度。
