Thompson S J, Whitley H J, Naysmith J D, Carswell F
Department of Child Health, University of Bristol, U.K.
Immunology. 1988 Jun;64(2):311-4.
An aqueous house dust mite extract was separated by SDS-PAGE and transferred to nitrocellulose membranes by Western Blotting. Two major allergens, Der pI and Der pII associated with the mite faeces and body, respectively, were identified on the protein blot. The blot was then probed with atopic sera and the bound antibodies were labelled with 125I anti-IgE and visualized by autoradiography. Using this technique it is shown that serum samples from eczema patients contain a higher proportion of anti-mite body IgE antibodies, whereas those from asthma patients contain a higher proportion of antibodies against the major mite faecal allergen Der pI. Minor mite body allergens are also shown to be important in eczematous patients. The role of mite allergens in atopic conditions is discussed, along with the significance of the findings presented here.
用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离水性屋尘螨提取物，并通过蛋白质印迹法将其转移至硝酸纤维素膜上。在蛋白质印迹上鉴定出两种主要变应原，分别与螨粪便和躯体相关的Der pI和Der pII。然后用特应性血清探测该印迹，并用125I抗IgE标记结合的抗体，通过放射自显影进行可视化。使用该技术表明，湿疹患者的血清样本中抗螨躯体IgE抗体比例较高，而哮喘患者的血清样本中针对主要螨粪便变应原Der pI的抗体比例较高。次要的螨躯体变应原在湿疹患者中也显示出重要性。讨论了螨变应原在特应性疾病中的作用以及此处呈现结果的意义。
