Faulkner M
Liverpool University, Department of Environmental and Evolutionary Biology, Brownlow Hill, England.
Electrophoresis. 1989 Apr;10(4):260-4. doi: 10.1002/elps.1150100408.
Sodium dodecyl sulphate-polyacrylamide gel electrophoresis has been used to determine the metacercariae (Mc) total body protein band profiles of different Diplostomum spp. Four species of fish were investigated, roach (Rutilus rutilus) infected with D. spathaceum Mc, gywniad (Coregonus laveratus) infected with D. coregonus Mc, ruff (Gymnocephalus cernua) infected with a Diplostomum species related to D. gasterostei Mc, and perch (Perca fluviatilis) infected with D. gasterostei Mc. The four species of Diplostomum Mc were distinguished by three different bands of molecular weight, Mr 55,500, 53,500 and 52,000. A homology of polypeptide component distribution was evident for D. gasterostei Mc, from P. fluviatilis, and D. coregonus Mc, from C. laveratus, the latter showing reduced protein concentrations between bands Mr 32,000-40,000. Critical analysis of Mc polypeptide patterns showed no evidence of contamination with lens, retina or vitreous humour host eye protein. Gross morphological data for the parasites was also considered in relation to different band profiles obtained for the Diplostomum spp. Mc. Band profile analysis in conjunction with other taxonomic tests proved to be a useful tool in the identification of unknown species.
十二烷基硫酸钠-聚丙烯酰胺凝胶电泳已被用于测定不同双穴吸虫属物种的尾蚴（Mc）全身蛋白条带图谱。研究了四种鱼类，感染了匙形双穴吸虫尾蚴的拟鲤（Rutilus rutilus）、感染了湖双穴吸虫尾蚴的吉氏白鲑（Coregonus laveratus）、感染了与腹口双穴吸虫尾蚴相关的双穴吸虫物种的杜父鱼（Gymnocephalus cernua）以及感染了腹口双穴吸虫尾蚴的鲈鱼（Perca fluviatilis）。这四种双穴吸虫尾蚴可通过分子量分别为55,500、53,500和52,000的三条不同条带加以区分。来自鲈鱼的腹口双穴吸虫尾蚴和来自吉氏白鲑的湖双穴吸虫尾蚴在多肽成分分布上具有同源性，后者在分子量32,000 - 40,000的条带之间蛋白质浓度降低。对尾蚴多肽模式的严格分析表明，没有证据显示受到晶状体、视网膜或玻璃体宿主眼蛋白的污染。还结合双穴吸虫属尾蚴获得的不同条带图谱考虑了寄生虫的总体形态学数据。条带图谱分析与其他分类学测试相结合被证明是鉴定未知物种的有用工具。
