Devos K M, Vercruysse-Dewitte D
Laboratory of Physiological Chemistry, University of Gent, Belgium.
Electrophoresis. 1989 Apr;10(4):267-8. doi: 10.1002/elps.1150100410.
A method was developed for the preparation of completely intact plant DNA embedded in agarose, and suitable for restriction enzyme digestion. Digestion with restriction enzyme was carried out according to modified protocols of Anand and Kenwrick et al. The new method of DNA isolation allows the separation of high molecular weight plant DNA by pulsed field gel electrophoresis.
开发了一种制备包埋在琼脂糖中且适合限制性酶切消化的完全完整植物DNA的方法。根据阿南德和肯里克等人修改后的方案，用限制性酶进行消化。新的DNA分离方法允许通过脉冲场凝胶电泳分离高分子量植物DNA。
