Thie M, Schlumberger W, Rauterberg J, Robenek H
Medizinische Fakultät der Universität, Arbeitsgruppe Zellbiologie, Münster/Bundesrepublik Deutschland.
Eur J Cell Biol. 1989 Apr;48(2):294-302.
Collagen synthesis in fibroblasts cultivated in collagen lattices is known to be repressed compared to synthesis in monolayer cultures on plastic. Inhibition of synthesis is supposed to be due to interactions between the plasma membrane and adjacent collagen fibrils. To evaluate how collagen synthesis is regulated in gel-cultured cells we cultivated fibroblasts within gels of polymerized alginate in which preexisting extracellular matrix components, e.g., type I collagen fibrils, were lacking. When alginate gels were examined at the ultrastructural level, normal collagen fibrils were not observed. However, broad sheets of microfibrillar material and so-called zebra bodies were found. The amount of collagen synthesized by fibroblasts in calcium alginate gels remained constant during the entire culture time and was about 70% of that produced in monolayer-cultured cells. This value corresponded to levels found in fully retracted collagen lattices on day 7 of culture. Our data suggest that interactions between the plasma membrane and adjacent collagen fibrils are not necessary for the inhibition of collagen synthesis. Thus, we present data that mechanical confinement is capable of inhibiting collagen synthesis in fibroblasts.
与在塑料上的单层培养中相比，已知在胶原晶格中培养的成纤维细胞中的胶原合成受到抑制。合成的抑制被认为是由于质膜与相邻胶原纤维之间的相互作用。为了评估在凝胶培养的细胞中胶原合成是如何被调节的，我们在聚合藻酸盐凝胶中培养成纤维细胞，其中缺乏预先存在的细胞外基质成分，例如I型胶原纤维。当在超微结构水平检查藻酸盐凝胶时，未观察到正常的胶原纤维。然而，发现了宽片状的微纤维材料和所谓的斑马体。在整个培养期间，成纤维细胞在海藻酸钙凝胶中合成的胶原量保持恒定，约为单层培养细胞中产生量的70%。该值与培养第7天完全收缩的胶原晶格中的水平相当。我们的数据表明，质膜与相邻胶原纤维之间的相互作用对于抑制胶原合成不是必需的。因此，我们提供的数据表明机械限制能够抑制成纤维细胞中的胶原合成。
