Mayes M D, Walker E R, Frederickson R G, Prince R K, DiBartolomeo A G
Department of Medicine, Wayne State University School of Medicine, Detroit, MI.
J Rheumatol. 1988 Feb;15(2):268-75.
Fibroblasts from normal and progressive systemic sclerosis involved skin more closely resemble in vivo cells when cultured in a collagen gel matrix, than do fibroblasts cultured on plastic. Ultrastructural studies show that the cytoskeleton and secretory organelles from these cells are better developed in the presence of a collagen gel. Fibronectin, glycosaminoglycans and collagen fibrils are produced by the cells and deposited in the preformed matrix. Collagen synthesis is greater in the scleroderma derived fibroblasts for all culture conditions with increased deposition in the matrix. Total collagen production form cells associated with the gel is less than that from those cultured on plastic, suggesting an inhibition of collagen synthesis by the preformed collagen matrix.
与在塑料上培养的成纤维细胞相比，正常皮肤和进行性系统性硬化症受累皮肤的成纤维细胞在胶原凝胶基质中培养时更类似于体内细胞。超微结构研究表明，在存在胶原凝胶的情况下，这些细胞的细胞骨架和分泌细胞器发育得更好。细胞产生纤连蛋白、糖胺聚糖和胶原纤维，并沉积在预先形成的基质中。在所有培养条件下，硬皮病来源的成纤维细胞中的胶原合成都更多，且在基质中的沉积增加。与凝胶相关的细胞产生的总胶原量少于在塑料上培养的细胞，这表明预先形成的胶原基质对胶原合成有抑制作用。
