Marasini Nirmal, Ghaffar Khairunnisa Abdul, Giddam Ashwini Kumar, Batzloff Michael R, Good Michael F, Skwarczynski Mariusz, Toth Istvan
The University of Queensland, School of Chemistry & Molecular Biosciences, St Lucia, QLD, 4072, Australia.
Griffith University, Institute for Glycomics, Gold Coast, QLD, 4222, Australia.
Curr Drug Deliv. 2017;14(5):701-708. doi: 10.2174/1567201813666160721141322.
Group A streptococcus (GAS) primarily colonizes the mucosal region of the upper respiratory tract, slowly leading to systemic infections. Thus, GAS-specific antibody responses are desirable at mucosal sites for early prevention against GAS colonization.
Herein, we developed a potent nanoliposomes-based delivery system for mucosally active lipid core peptide (LCP)-based vaccines.
Trimethyl chitosan (TMC)-coated liposomes that bore a B-cell epitope derived from GAS Mprotein, stimulated potent epitope-specific mucosal and systemic antibody titres after only one boost following intranasal immunization in Swiss outbred mice. The immune responses were durable even at day 139 post-primary immunization.
The enhanced vaccine efficacy, lowered dose, and simple and cost-effective process of producing the coated nanoliposomes should be particularly useful in developing potent peptide-based vaccines to prevent infections at the mucosal sites.
A 组链球菌（GAS）主要在上呼吸道黏膜区域定植，逐渐导致全身感染。因此，在黏膜部位产生 GAS 特异性抗体反应对于早期预防 GAS 定植是可取的。
在此，我们开发了一种基于纳米脂质体的高效递送系统，用于基于黏膜活性脂质核心肽（LCP）的疫苗。
包被有源自 GAS M 蛋白的 B 细胞表位的三甲基壳聚糖（TMC）包被脂质体，在瑞士远交小鼠鼻内免疫仅一次加强免疫后，刺激产生了强效的表位特异性黏膜和全身抗体滴度。即使在初次免疫后第 139 天，免疫反应仍然持久。
增强的疫苗效力、降低的剂量以及生产包被纳米脂质体的简单且经济高效的过程，在开发强效的基于肽的疫苗以预防黏膜部位感染方面应特别有用。
