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大鼠延髓中缝核内同时含有5-羟色胺和脑啡肽免疫反应性的单个细胞投射至脊髓。

Individual cells in the raphe nuclei of the medulla oblongata in rat that contain immunoreactivities for both serotonin and enkephalin project to the spinal cord.

作者信息

Millhorn D E, Hökfelt T, Verhofstad A A, Terenius L

机构信息

Department of Physiology, University of North Carolina, Chapel Hill 27599.

出版信息

Exp Brain Res. 1989;75(3):536-42. doi: 10.1007/BF00249904.

DOI:10.1007/BF00249904
PMID:2744110
Abstract

The ventral medulla oblongata of rats was analyzed with a double-labelling immunofluorescence technique using guinea pig antibodies directed against serotonin (5-HT) and rabbit antisera directed against enkephalin (ENK). Numerous cells in the region of nucleus raphe obscurus, nucleus raphe pallidus and nucleus raphe magnus showed immunostaining for either 5-HT or ENK. A substantial number of cells showed positive immunostaining for both 5-HT and ENK. 5-HT/ENK double-labelled cells were most frequently encountered in an area that extended from the rostral aspect of the inferior olivary nucleus to the pontomedullary border. This region corresponds anatomically to nucleus raphe magnus/nucleus paragigantocellularis. In addition, a number of the 5-HT/ENK-containing cells were retrogradely labelled with Fluoro-Gold dye that had been injected into the thoracic spinal cord several days prior to perfusion. Schematic drawings showing the anatomical distribution of 5-HT/ENK colocalization are provided.

摘要

采用双标记免疫荧光技术，用豚鼠抗血清针对5-羟色胺（5-HT）以及兔抗血清针对脑啡肽（ENK），对大鼠延髓腹侧进行分析。在中缝隐核、中缝苍白核和中缝大核区域的许多细胞，显示出5-HT或ENK的免疫染色。大量细胞对5-HT和ENK均显示阳性免疫染色。5-HT/ENK双标记细胞最常见于从下橄榄核嘴侧延伸至脑桥延髓边界的区域。该区域在解剖学上对应于中缝大核/巨细胞旁核。此外，一些含5-HT/ENK的细胞被逆行注射到灌注前几天注入胸段脊髓的荧光金染料标记。提供了显示5-HT/ENK共定位解剖分布的示意图。

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